Please use this identifier to cite or link to this item:
https://doi.org/10.1186/1471-2164-12-509
Title: | Gene expression profiling in the Cynomolgus macaque Macaca fascicularis shows variation within the normal birth range | Authors: | Emerald, B.S Chng, K Masuda, S Sloboda, D.M Vickers, M.H Kambadur, R Gluckman, P.D |
Keywords: | carbohydrate lipid nitrogen nucleic acid animal tissue article biological activity biosynthesis carbohydrate metabolism catabolism cell adhesion cell maturation cell metabolism controlled study DNA microarray female fetus development gene expression profiling gene function gene identification gene location genetic variability lipid metabolism liver low birth weight Macaca fascicularis male metabolic regulation newborn nitrogen metabolism nonhuman nucleic acid metabolism nucleotide sequence skeletal muscle tissue distribution umbilical cord unindexed sequence animal birth weight genetics metabolism pregnancy Animalia Macaca fascicularis Primates Animals Animals, Newborn Birth Weight Female Gene Expression Profiling Liver Macaca fascicularis Muscle, Skeletal Oligonucleotide Array Sequence Analysis Pregnancy Umbilical Cord |
Issue Date: | 2011 | Citation: | Emerald, B.S, Chng, K, Masuda, S, Sloboda, D.M, Vickers, M.H, Kambadur, R, Gluckman, P.D (2011). Gene expression profiling in the Cynomolgus macaque Macaca fascicularis shows variation within the normal birth range. BMC Genomics 12 : 509. ScholarBank@NUS Repository. https://doi.org/10.1186/1471-2164-12-509 | Rights: | Attribution 4.0 International | Abstract: | Background: Although an adverse early-life environment has been linked to an increased risk of developing the metabolic syndrome, the molecular mechanisms underlying altered disease susceptibility as well as their relevance to humans are largely unknown. Importantly, emerging evidence suggests that these effects operate within the normal range of birth weights and involve mechanisms of developmental palsticity rather than pathology.Method: To explore this further, we utilised a non-human primate model Macaca fascicularis (Cynomolgus macaque) which shares with humans the same progressive history of the metabolic syndrome. Using microarray we compared tissues from neonates in the average birth weight (50-75thcentile) to those of lower birth weight (5-25thcentile) and studied the effect of different growth trajectories within the normal range on gene expression levels in the umbilical cord, neonatal liver and skeletal muscle.Results: We identified 1973 genes which were differentially expressed in the three tissue types between average and low birth weight animals (P < 0.05). Gene ontology analysis identified that these genes were involved in metabolic processes including cellular lipid metabolism, cellular biosynthesis, cellular macromolecule synthesis, cellular nitrogen metabolism, cellular carbohydrate metabolism, cellular catabolism, nucleotide and nucleic acid metabolism, regulation of molecular functions, biological adhesion and development.Conclusion: These differences in gene expression levels between animals in the upper and lower percentiles of the normal birth weight range may point towards early life metabolic adaptations that in later life result in differences in disease risk. © 2011 Emerald et al; licensee BioMed Central Ltd. | Source Title: | BMC Genomics | URI: | https://scholarbank.nus.edu.sg/handle/10635/181625 | ISSN: | 14712164 | DOI: | 10.1186/1471-2164-12-509 | Rights: | Attribution 4.0 International |
Appears in Collections: | Elements Staff Publications |
Show full item record
Files in This Item:
File | Description | Size | Format | Access Settings | Version | |
---|---|---|---|---|---|---|
10_1186_1471-2164-12-509.pdf | 1.38 MB | Adobe PDF | OPEN | None | View/Download |
This item is licensed under a Creative Commons License