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https://doi.org/10.1186/1471-2334-13-90
Title: | High prevalence of CXCR4 usage among treatment-naive CRF01_AE and CRF51_01B-infected HIV-1 subjects in Singapore | Authors: | Ng, K.Y Chew, K.K Kaur, P Kwan, J.Y Khong, W.X Lin, L Chua, A Tan, M.T Quinn, T.C Laeyendecker, O Leo, Y.S Ng, O.T |
Keywords: | chemokine receptor CXCR4 glycoprotein gp 120 glycoprotein gp 41 Pol protein adult article controlled study female genotype human Human immunodeficiency virus 1 infection major clinical study male sequence analysis Singapore viral tropism virus classification virus resistance Adolescent Adult Child Female Genotype HIV Envelope Protein gp120 HIV Infections HIV-1 Humans Male Middle Aged Prevalence Receptors, CCR5 Receptors, CXCR4 Singapore Viral Tropism Young Adult |
Issue Date: | 2013 | Citation: | Ng, K.Y, Chew, K.K, Kaur, P, Kwan, J.Y, Khong, W.X, Lin, L, Chua, A, Tan, M.T, Quinn, T.C, Laeyendecker, O, Leo, Y.S, Ng, O.T (2013). High prevalence of CXCR4 usage among treatment-naive CRF01_AE and CRF51_01B-infected HIV-1 subjects in Singapore. BMC Infectious Diseases 13 (1) : 90. ScholarBank@NUS Repository. https://doi.org/10.1186/1471-2334-13-90 | Rights: | Attribution 4.0 International | Abstract: | Background: Recent studies suggest HIV-1 inter-subtype differences in co-receptor usage. We examined the correlation between HIV-1 subtype and co-receptor usage among treatment-naïve HIV-1 subjects in Singapore. Additionally, we investigated whether the subtype co-receptor association was influenced by stage of infection.Methods: V3 sequences of HIV-1 envelope protein gp120 were obtained from 110 HIV treatment-naïve patients and genotypic co-receptor tropism determination was performed using Geno2pheno. Two false-positive rate (FPR) cut-offs, 10% and 5.75% were selected for tropism testing.Results: Subtype assignment of viral strains from 110 HIV-infected individuals based on partial sequencing of HIV-1 pol, gp120 and gp41 were as follows: 27 subtype B, 64 CRF01_AE, 10 CRF51_01B, and 9 other subtypes. At FPR=10%, 10 (100%) CRF51_01B-infected subjects and 26 (40.6%) CRF01_AE-infected subjects had CXCR4-using virus, compared to 7 (25.9%) subtype B subjects and 1 (11.1%) CRF33_01B-infected subject (P < 0.001). At FPR=5.75%, 10 (100%) CRF51_01B-infected subjects and 20 (31.3%) CRF01_AE-infected subjects had CXCR4-using virus, compared to 4 (14.8%) subtype B and 1 (11.1%) CRF33_01B-infected subjects (P < 0.001). Among those with evidence of seroconversion within 2 years prior to study enrolment, 100% of CRF51_01B-infected subjects had CXCR4-using virus, independent of Geno2pheno FPR.Conclusion: CRF51_01B and CRF01_AE-infected individuals have higher prevalence of CXCR4-usage compared to subtype B infected individuals. Further studies examining these differences could help optimise the use of CCR5-antagonist in populations with these subtypes, and increase our understanding of HIV-1 biology. © 2013 Ng et al; licensee BioMed Central Ltd. | Source Title: | BMC Infectious Diseases | URI: | https://scholarbank.nus.edu.sg/handle/10635/181579 | ISSN: | 14712334 | DOI: | 10.1186/1471-2334-13-90 | Rights: | Attribution 4.0 International |
Appears in Collections: | Elements Staff Publications |
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