Please use this identifier to cite or link to this item: https://doi.org/10.1186/1471-2334-13-90
Title: High prevalence of CXCR4 usage among treatment-naive CRF01_AE and CRF51_01B-infected HIV-1 subjects in Singapore
Authors: Ng, K.Y
Chew, K.K
Kaur, P
Kwan, J.Y
Khong, W.X
Lin, L
Chua, A
Tan, M.T
Quinn, T.C
Laeyendecker, O
Leo, Y.S 
Ng, O.T 
Keywords: chemokine receptor CXCR4
glycoprotein gp 120
glycoprotein gp 41
Pol protein
adult
article
controlled study
female
genotype
human
Human immunodeficiency virus 1 infection
major clinical study
male
sequence analysis
Singapore
viral tropism
virus classification
virus resistance
Adolescent
Adult
Child
Female
Genotype
HIV Envelope Protein gp120
HIV Infections
HIV-1
Humans
Male
Middle Aged
Prevalence
Receptors, CCR5
Receptors, CXCR4
Singapore
Viral Tropism
Young Adult
Issue Date: 2013
Citation: Ng, K.Y, Chew, K.K, Kaur, P, Kwan, J.Y, Khong, W.X, Lin, L, Chua, A, Tan, M.T, Quinn, T.C, Laeyendecker, O, Leo, Y.S, Ng, O.T (2013). High prevalence of CXCR4 usage among treatment-naive CRF01_AE and CRF51_01B-infected HIV-1 subjects in Singapore. BMC Infectious Diseases 13 (1) : 90. ScholarBank@NUS Repository. https://doi.org/10.1186/1471-2334-13-90
Rights: Attribution 4.0 International
Abstract: Background: Recent studies suggest HIV-1 inter-subtype differences in co-receptor usage. We examined the correlation between HIV-1 subtype and co-receptor usage among treatment-naïve HIV-1 subjects in Singapore. Additionally, we investigated whether the subtype co-receptor association was influenced by stage of infection.Methods: V3 sequences of HIV-1 envelope protein gp120 were obtained from 110 HIV treatment-naïve patients and genotypic co-receptor tropism determination was performed using Geno2pheno. Two false-positive rate (FPR) cut-offs, 10% and 5.75% were selected for tropism testing.Results: Subtype assignment of viral strains from 110 HIV-infected individuals based on partial sequencing of HIV-1 pol, gp120 and gp41 were as follows: 27 subtype B, 64 CRF01_AE, 10 CRF51_01B, and 9 other subtypes. At FPR=10%, 10 (100%) CRF51_01B-infected subjects and 26 (40.6%) CRF01_AE-infected subjects had CXCR4-using virus, compared to 7 (25.9%) subtype B subjects and 1 (11.1%) CRF33_01B-infected subject (P < 0.001). At FPR=5.75%, 10 (100%) CRF51_01B-infected subjects and 20 (31.3%) CRF01_AE-infected subjects had CXCR4-using virus, compared to 4 (14.8%) subtype B and 1 (11.1%) CRF33_01B-infected subjects (P < 0.001). Among those with evidence of seroconversion within 2 years prior to study enrolment, 100% of CRF51_01B-infected subjects had CXCR4-using virus, independent of Geno2pheno FPR.Conclusion: CRF51_01B and CRF01_AE-infected individuals have higher prevalence of CXCR4-usage compared to subtype B infected individuals. Further studies examining these differences could help optimise the use of CCR5-antagonist in populations with these subtypes, and increase our understanding of HIV-1 biology. © 2013 Ng et al; licensee BioMed Central Ltd.
Source Title: BMC Infectious Diseases
URI: https://scholarbank.nus.edu.sg/handle/10635/181579
ISSN: 14712334
DOI: 10.1186/1471-2334-13-90
Rights: Attribution 4.0 International
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