Please use this identifier to cite or link to this item: https://doi.org/10.3390/md11051492
Title: A shark liver gene-derived active peptide expressed in the silkworm, Bombyx mori: Preliminary studies for oral administration of the recombinant protein
Authors: Liu, Y
Chen, Y
Chen, J
Zhang, W
Sheng, Q
Chen, J
Yu, W
Nie, Z
Zhang, Y
Wu, W
Wang, L
Indran, I.R 
Li, J
Qian, L
Lv, Z
Keywords: glucose
recombinant active peptide from shark liver
recombinant cytokine
unclassified drug
animal cell
animal experiment
animal model
animal tissue
antidiabetic activity
article
Bombyx mori
controlled study
drug effect
drug efficacy
gene expression
glucose blood level
glucose metabolism
male
molecular cloning
mouse
non insulin dependent diabetes mellitus
nonhuman
pancreas islet
powder
streptozocin diabetes
Administration, Oral
Animals
Blood Glucose
Bombyx
Cytokines
Diabetes Mellitus, Experimental
Diabetes Mellitus, Type 2
Islets of Langerhans
Liver
Male
Mice
Mice, Inbred ICR
Powders
Pupa
Recombinant Proteins
Sharks
Streptozocin
Animalia
Bombyx mori
Chiloscyllium plagiosum
Chondrichthyes
Mus
Issue Date: 2013
Citation: Liu, Y, Chen, Y, Chen, J, Zhang, W, Sheng, Q, Chen, J, Yu, W, Nie, Z, Zhang, Y, Wu, W, Wang, L, Indran, I.R, Li, J, Qian, L, Lv, Z (2013). A shark liver gene-derived active peptide expressed in the silkworm, Bombyx mori: Preliminary studies for oral administration of the recombinant protein. Marine Drugs 11 (5) : 1492-1505. ScholarBank@NUS Repository. https://doi.org/10.3390/md11051492
Rights: Attribution 4.0 International
Abstract: Active peptide from shark liver (APSL) is a cytokine from Chiloscyllium plagiosum that can stimulate liver regeneration and protects the pancreas. To study the effect of orally administered recombinant APSL (rAPSL) on an animal model of type 2 diabetes mellitus, the APSL gene was cloned, and APSL was expressed in Bombyx mori N cells (BmN cells), silkworm larvae and silkworm pupae using the silkworm baculovirus expression vector system (BEVS). It was demonstrated that rAPSL was able to significantly reduce the blood glucose level in mice with type 2 diabetes induced by streptozotocin. The analysis of paraffin sections of mouse pancreatic tissues revealed that rAPSL could effectively protect mouse islets from streptozotocin-induced lesions. Compared with the powder prepared from normal silkworm pupae, the powder prepared from pupae expressing rAPSL exhibited greater protective effects, and these results suggest that rAPSL has potential uses as an oral drug for the treatment of diabetes mellitus in the future. © 2013 by the authors licensee MDPI.
Source Title: Marine Drugs
URI: https://scholarbank.nus.edu.sg/handle/10635/181571
ISSN: 16603397
DOI: 10.3390/md11051492
Rights: Attribution 4.0 International
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