Please use this identifier to cite or link to this item: https://doi.org/10.1186/1471-2180-13-148
Title: Cis-2-dodecenoic acid quorum sensing system modulates N-acyl homoserine lactone production through RpfR and cyclic di-GMP turnover in Burkholderia cenocepacia
Authors: Deng, Y
Lim, A
Wang, J
Zhou, T
Chen, S
Lee, J
Dong, Y.-H
Zhang, L.-H 
Keywords: bacterial protein
cyclic GMP
n acylhomoserine lactone
RpfR protein
unclassified drug
article
bacterial genetics
bacterial mutation
bacterial virulence
biofilm
biological functions
Burkholderia cenocepacia
deletion mutant
down regulation
genetic analysis
nonhuman
phenotype
quorum sensing
regulatory mechanism
second messenger
signal transduction
turnover time
4-Butyrolactone
Bacterial Proteins
Burkholderia cenocepacia
Cyclic GMP
Down-Regulation
Fatty Acids, Monounsaturated
Gene Expression Regulation, Bacterial
Quorum Sensing
Signal Transduction
Bacteria (microorganisms)
Burkholderia cenocepacia
Issue Date: 2013
Citation: Deng, Y, Lim, A, Wang, J, Zhou, T, Chen, S, Lee, J, Dong, Y.-H, Zhang, L.-H (2013). Cis-2-dodecenoic acid quorum sensing system modulates N-acyl homoserine lactone production through RpfR and cyclic di-GMP turnover in Burkholderia cenocepacia. BMC Microbiology 13 (1) : 148. ScholarBank@NUS Repository. https://doi.org/10.1186/1471-2180-13-148
Rights: Attribution 4.0 International
Abstract: Background: Burkholderia cenocepacia employs both N-Acyl homoserine lactone (AHL) and cis-2-dodecenoic acid (BDSF) quorum sensing (QS) systems in regulation of bacterial virulence. It was shown recently that disruption of BDSF synthase RpfF§ssub§Bc§esub§ caused a reduction of AHL signal production in B. cenocepacia. However, how BDSF system influences AHL system is still not clear. Results: We show here that BDSF system controls AHL system through a novel signaling mechanism. Null mutation of either the BDSF synthase, RpfF§ssub§Bc§esub§, or the BDSF receptor, RpfR, caused a substantial down-regulation of AHL signal production in B. cenocepacia strain H111. Genetic and biochemical analyses showed that BDSF system controls AHL signal production through the transcriptional regulation of the AHL synthase gene cepI by modulating the intracellular level of second messenger cyclic di-GMP (c-di-GMP). Furthermore, we show that BDSF and AHL systems have a cumulative role in the regulation of various biological functions, including swarming motility, biofilm formation and virulence factor production, and exogenous addition of either BDSF or AHL signal molecules could only partially rescue the changed phenotypes of the double deletion mutant defective in BDSF and AHL signal production. Conclusions: These results, together with our previous findings, thus depict a molecular mechanism with which BDSF regulates AHL signal production and bacterial virulence through modulating the phosphodiesterase activity of its receptor RpfR to influence the intracellular level of c-di-GMP. © 2013 Deng et al.; licensee BioMed Central Ltd.
Source Title: BMC Microbiology
URI: https://scholarbank.nus.edu.sg/handle/10635/181565
ISSN: 14712180
DOI: 10.1186/1471-2180-13-148
Rights: Attribution 4.0 International
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