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https://doi.org/10.1002/jssc.201600172
Title: | Quantitative analysis of N-acylphosphatidylethanolamine molecular species in rat brain using solid-phase extraction combined with reversed-phase chromatography and tandem mass spectrometry | Authors: | Triebl, A Weissengruber, S Trötzmüller, M Lankmayr, E Köfeler, H |
Keywords: | Chains Chemical analysis Chromatography High performance liquid chromatography Liquid chromatography Liquids Mass spectrometry Molecular structure Phase separation Rats Spectrometry Chromatographic separations Electrospray tandem mass spectrometry Lower limit of quantifications N-acylphosphatidylethanolamines Reversed phase chromatography Reversed phase high performance liquid chromatography Selected reaction monitoring Solid-phase extraction Extraction phosphatidylethanolamine analytic method Article brain level limit of quantitation measurement accuracy nonhuman priority journal quantitative analysis rat reversed phase liquid chromatography solid phase extraction tandem mass spectrometry validation process |
Issue Date: | 2016 | Citation: | Triebl, A, Weissengruber, S, Trötzmüller, M, Lankmayr, E, Köfeler, H (2016). Quantitative analysis of N-acylphosphatidylethanolamine molecular species in rat brain using solid-phase extraction combined with reversed-phase chromatography and tandem mass spectrometry. Journal of Separation Science 39 (13) : 2474-2480. ScholarBank@NUS Repository. https://doi.org/10.1002/jssc.201600172 | Rights: | Attribution 4.0 International | Abstract: | A novel method for the sensitive and selective identification and quantification of N-acylphosphatidylethanolamine molecular species was developed. Samples were prepared using a combination of liquid–liquid and solid-phase extraction, and intact N-acylphosphatidylethanolamine species were determined by reversed-phase high-performance liquid chromatography coupled to positive electrospray tandem mass spectrometry. As a result of their biological functions as precursors for N-acylethanolamines and as signaling molecules, tissue concentrations of N-acylphosphatidylethanolamines are very low, and their analysis is additionally hindered by the vast excess of other sample components. Our sample preparation methods are able to selectively separate the analytes of interest from any expected biological interferences. Finally, the highest selectivity is achieved by coupling chromatographic separation and two N-acyl chain specific selected reaction monitoring scans per analyte, enabling identification of both the N-acyl chain and the phosphatidylethanolamine moiety. The validated method is suitable for the reliable quantification of N-acylphosphatidylethanolamine species from rat brain with a lower limit of quantification of 10 pmol/g and a linear range up to 2300 pmol/g. In total, 41 N-acylphosphatidylethanolamine molecular species with six different N-acyl chains, amounting to a total concentration of 3 nmol/g, were quantified. © 2016 The Authors, Journal of Separation Science Published by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim | Source Title: | Journal of Separation Science | URI: | https://scholarbank.nus.edu.sg/handle/10635/181359 | ISSN: | 16159306 | DOI: | 10.1002/jssc.201600172 | Rights: | Attribution 4.0 International |
Appears in Collections: | Elements Staff Publications |
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