Please use this identifier to cite or link to this item: https://doi.org/10.1186/s12906-017-1635-1
Title: Vaccaria hypaphorine alleviates lipopolysaccharide-induced inflammation via inactivation of NF?B and ERK pathways in Raw 264.7 cells
Authors: Sun, H 
Cai, W
Wang, X
Liu, Y
Hou, B
Zhu, X
Qiu, L
Keywords: acetylsalicylic acid
antiinflammatory agent
cyclooxygenase 2
dexamethasone
herbaceous agent
I kappa B kinase alpha
I kappa B kinase beta
immunoglobulin enhancer binding protein
inducible nitric oxide synthase
interleukin 10
interleukin 1beta
interleukin 6
lipopolysaccharide
messenger RNA
mitogen activated protein kinase
monocyte chemotactic protein 1
nitric oxide
prostaglandin E2
transcription factor RelA
tumor necrosis factor
unclassified drug
Vaccaria hypaphorine
antiinflammatory agent
autacoid
cytokine
I kappa B kinase
I kappa B kinase alpha
immunoglobulin enhancer binding protein
indole derivative
lenticin
nitric oxide
plant extract
animal cell
antiinflammatory activity
Article
cell viability
cellular distribution
concentration response
controlled study
cytokine production
down regulation
drug effect
drug mechanism
enzyme inhibition
enzyme phosphorylation
gene expression regulation
human
human cell
immunofluorescence
in vitro study
inflammation
intracellular signaling
lipopolysaccharide induced inflammation
MCF-7 cell line
molecular dynamics
mouse
nonhuman
outcome assessment
RAW 264.7 cell line
animal
chemically induced
chemistry
drug effects
inflammation
macrophage
metabolism
phosphorylation
phytotherapy
RAW 264.7 cell line
signal transduction
transport at the cellular level
Vaccaria
Animals
Anti-Inflammatory Agents
Biological Transport
Cyclooxygenase 2
Cytokines
Humans
I-kappa B Kinase
Indoles
Inflammation
Inflammation Mediators
Lipopolysaccharides
Macrophages
MAP Kinase Signaling System
MCF-7 Cells
Mice
NF-kappa B
NF-KappaB Inhibitor alpha
Nitric Oxide
Nitric Oxide Synthase Type II
Phosphorylation
Phytotherapy
Plant Extracts
RAW 264.7 Cells
Vaccaria
Issue Date: 2017
Citation: Sun, H, Cai, W, Wang, X, Liu, Y, Hou, B, Zhu, X, Qiu, L (2017). Vaccaria hypaphorine alleviates lipopolysaccharide-induced inflammation via inactivation of NF?B and ERK pathways in Raw 264.7 cells. BMC Complementary and Alternative Medicine 17 (1) : 120. ScholarBank@NUS Repository. https://doi.org/10.1186/s12906-017-1635-1
Rights: Attribution 4.0 International
Abstract: Background: Activation of macrophage is involved in many inflammation diseases. Lipopolysaccharide (LPS) is a powerful inflammatory signal contributing to monocytes/macrophages activation associated with increased proinflammatory cytokines expressions. We recently identified that vaccarin was expected to protect endothelial cells from injury. Hypaphorine was abundantly found in vaccaria semen. However, the potential roles and underlying mechanisms of vaccaria hypaphorine on macrophage inflammation have been poorly defined. Methods: This study was designed to determine the effects of vaccaria hypaphorine on LPS-mediated inflammation in RAW 264.7 cells. Results: In this study, we demonstrated that vaccaria hypaphorine dramatically ameliorated LPS-induced nitric oxide (NO) release and productions of proinflammatory cytokines including tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), IL-6, IL-10, monocyte chemoattractant protein 1 (MCP-1) and prostaglandin E2 (PGE2) in RAW 264.7 cells. LPS-stimulated expressions of cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) were down-regulated by vaccaria hypaphorine. Furthermore, vaccaria hypaphorine retarded LPS-induced phosphorylation of ERK, nuclear factor kappa beta (NFΚB), NFΚB inhibitor IΚBα, and IKKβ. Immunofluorescence staining revealed that vaccaria hypaphorine eliminated the nuclear translocation of NFΚB in LPS-treated RAW 264.7 cells. Conclusion: It was seen that vaccaria hypaphorine counteracted inflammation via inhibition of ERK or/and NFΚB signaling pathways. Collectively, we concluded that vaccaria hypaphorine can be served as an anti-inflammatory candidate. © 2017 The Author(s).
Source Title: BMC Complementary and Alternative Medicine
URI: https://scholarbank.nus.edu.sg/handle/10635/181298
ISSN: 14726882
DOI: 10.1186/s12906-017-1635-1
Rights: Attribution 4.0 International
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