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Title: Overexpression of OsERF48 causes regulation of OsCML16, a calmodulin-like protein gene that enhances root growth and drought tolerance
Authors: Jung, H 
Chung, P.J
Park, S.-H
Redillas, M.C.F.R
Kim, Y.S
Suh, J.-W
Kim, J.-K
Keywords: plant protein
transcription factor
amino acid sequence
calcium signaling
gene expression regulation
gene regulatory network
growth, development and aging
nucleotide sequence
physiological stress
plant root
promoter region
transgenic plant
Amino Acid Sequence
Base Sequence
Calcium Signaling
Gene Expression Regulation, Plant
Gene Regulatory Networks
Plant Proteins
Plant Roots
Plants, Genetically Modified
Promoter Regions, Genetic
Stress, Physiological
Transcription Factors
Issue Date: 2017
Citation: Jung, H, Chung, P.J, Park, S.-H, Redillas, M.C.F.R, Kim, Y.S, Suh, J.-W, Kim, J.-K (2017). Overexpression of OsERF48 causes regulation of OsCML16, a calmodulin-like protein gene that enhances root growth and drought tolerance. Plant Biotechnology Journal 15 (10) : 1295-1308. ScholarBank@NUS Repository.
Rights: Attribution 4.0 International
Abstract: The AP2/ERF family is a plant-specific transcription factor family whose members have been associated with various developmental processes and stress tolerance. Here, we functionally characterized the drought-inducible OsERF48, a group Ib member of the rice ERF family with four conserved motifs, CMI-1, -2, -3 and -4. A transactivation assay in yeast revealed that the C-terminal CMI-1 motif was essential for OsERF48 transcriptional activity. When OsERF48 was overexpressed in an either a root-specific (ROXO s ERF 48) or whole-body (OXO s ERF 48) manner, transgenic plants showed a longer and denser root phenotype compared to the nontransgenic (NT) controls. When plants were grown on a 40% polyethylene glycol-infused medium under in vitro drought conditions, ROXO s ERF 48 plants showed a more vigorous root growth than OXO s ERF 48 and NT plants. In addition, the ROXO s ERF 48 plants exhibited higher grain yield than OXO s ERF 48 and NT plants under field-drought conditions. We constructed a putative OsERF48 regulatory network by cross-referencing ROXO s ERF 48 root-specific RNA-seq data with a co-expression network database, from which we inferred the involvement of 20 drought-related genes in OsERF48-mediated responses. These included genes annotated as being involved in stress signalling, carbohydrate metabolism, cell-wall proteins and drought responses. They included, OsCML16, a key gene in calcium signalling during abiotic stress, which was shown to be a direct target of OsERF48 by chromatin immunoprecipitation-qPCR analysis and a transient protoplast expression assay. Our results demonstrated that OsERF48 regulates OsCML16, a calmodulin-like protein gene that enhances root growth and drought tolerance. © 2017 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd.
Source Title: Plant Biotechnology Journal
ISSN: 14677644
DOI: 10.1111/pbi.12716
Rights: Attribution 4.0 International
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