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Title: Partial genetic characterization of West Nile virus strains, New York State, 2000
Authors: Ebel, G.D
Dupuis II, A.P
Ngo, K
Nicholas, D
Kauffman, E
Jones, S.A
Young, D
Maffei, J
Shi, P.-Y 
Bernard, K
Kramer, L.D
Keywords: article
controlled study
DNA polymorphism
envelope gene
gene amplification
genetic analysis
nucleotide sequence
sequence analysis
sequence homology
strain difference
United States
viral genetics
virus isolation
virus transmission
West Nile flavivirus
Issue Date: 2001
Publisher: Centers for Disease Control and Prevention (CDC)
Citation: Ebel, G.D, Dupuis II, A.P, Ngo, K, Nicholas, D, Kauffman, E, Jones, S.A, Young, D, Maffei, J, Shi, P.-Y, Bernard, K, Kramer, L.D (2001). Partial genetic characterization of West Nile virus strains, New York State, 2000. Emerging Infectious Diseases 7 (4) : 650-653. ScholarBank@NUS Repository.
Rights: Attribution 4.0 International
Abstract: We analyzed nucleotide sequences from the envelope gene of 11 West Nile (WN) virus strains collected in New York State during the 2000 transmission season to determine whether they differed genetically from each other and from the initial strain isolated in 1999. The complete envelope genes of these strains were amplified by reverse transcription-polymerase chain reaction. The resulting sequences were aligned, the genetic distances were computed, and a phylogenetic tree was constructed. Ten (0.7%) of 1,503 positions in the envelope gene were polymorphic in one or more sequences. The genetic distances were 0.003 or less. WN virus strains circulating in 2000 were homogeneous with respect to one another and to a strain isolated in 1999.
Source Title: Emerging Infectious Diseases
ISSN: 10806040
DOI: 10.3201/eid0704.017408
Rights: Attribution 4.0 International
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