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Title: Synthesis and characterization of anti-HER2 antibody conjugated CdSe/CdZnS quantum dots for fluorescence imaging of breast cancer cells
Authors: Tiwari, D.K 
Tanaka, S.-I
Inouye, Y
Yoshizawa, K
Watanabe, T.M
Jin, T
Keywords: Breast cancer cells
Cellular imaging
Fluorescence Correlation Spectroscopy
Fluorescence quantum yield
Human breast cancer cells
Human epidermal growth factor
Synthesis and characterizations
Atomic force microscopy
Cell culture
Chemical detection
Coupling agents
Fluorescence spectroscopy
Light sources
Patient treatment
Semiconductor quantum dots
Spectroscopic analysis
Medical imaging
Issue Date: 2009
Citation: Tiwari, D.K, Tanaka, S.-I, Inouye, Y, Yoshizawa, K, Watanabe, T.M, Jin, T (2009). Synthesis and characterization of anti-HER2 antibody conjugated CdSe/CdZnS quantum dots for fluorescence imaging of breast cancer cells. Sensors 9 (11) : 9332-9354. ScholarBank@NUS Repository.
Rights: Attribution 4.0 International
Abstract: The early detection of HER2 (human epidermal growth factor receptor 2) status in breast cancer patients is very important for the effective implementation of anti-HER2 antibody therapy. Recently, HER2 detections using antibody conjugated quantum dots (QDs) have attracted much attention. QDs are a new class of fluorescent materials that have superior properties such as high brightness, high resistance to photo-bleaching, and multi-colored emission by a single-light source excitation. In this study, we synthesized three types of anti-HER2 antibody conjugated QDs (HER2Ab-QDs) using different coupling agents (EDC/sulfo-NHS, iminothiolane/sulfo-SMCC, and sulfo-SMCC). As water-soluble QDs for the conjugation of antibody, we used glutathione coated CdSe/CdZnS QDs (GSH-QDs) with fluorescence quantum yields of 0.23~0.39 in aqueous solution. Dispersibility, hydrodynamic size, and apparent molecular weights of the GSHQDs and HER2Ab-QDs were characterized by using dynamic light scattering, fluorescence correlation spectroscopy, atomic force microscope, and size-exclusion HPLC. Fluorescence imaging of HER2 overexpressing cells (KPL-4 human breast cancer cell line) was performed by using HER2Ab-QDs as fluorescent probes. We found that the HER2Ab-QD prepared by using SMCC coupling with partially reduced antibody is a most effective probe for the detection of HER2 expression in KPL-4 cells. We have also studied the size dependency of HER2Ab-QDs (with green, orange, and red emission) on the fluorescence image of KPL-4 cells. © 2009 by the authors.
Source Title: Sensors
ISSN: 14248220
DOI: 10.3390/s91109332
Rights: Attribution 4.0 International
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