Please use this identifier to cite or link to this item: https://doi.org/10.1128/IAI.00180-12
Title: A novel phage element of salmonella enterica serovar enteritidis p125109 contributes to accelerated type iii secretion system 2-dependent early inflammation kinetics in a mouse colitis model
Authors: Vishwakarma, V
Periaswamy, B 
Pati, N.B
Slack, E
Hardt, W
Suar, M
Keywords: green fluorescent protein
animal experiment
animal model
article
bacterial colonization
colitis
controlled study
fluorescence activated cell sorting
gene deletion
in vivo study
lamina propria
mesentery lymph node
mouse
nonhuman
polymerase chain reaction
priority journal
protein expression
Salmonella enteritidis
type III secretion system
Animals
Bacterial Secretion Systems
Colitis
Disease Models, Animal
Gene Deletion
Genetic Complementation Test
Humans
Mice
Mice, Inbred C57BL
Salmonella enteritidis
Salmonella Infections, Animal
Salmonella Phages
Salmonella typhimurium
Virulence
Virulence Factors
Issue Date: 2012
Citation: Vishwakarma, V, Periaswamy, B, Pati, N.B, Slack, E, Hardt, W, Suar, M (2012). A novel phage element of salmonella enterica serovar enteritidis p125109 contributes to accelerated type iii secretion system 2-dependent early inflammation kinetics in a mouse colitis model. Infection and Immunity 80 (9) : 3236-3246. ScholarBank@NUS Repository. https://doi.org/10.1128/IAI.00180-12
Rights: Attribution 4.0 International
Abstract: Salmonella enterica subsp. I serovar Enteritidis exhibits type III secretion system 2 (TTSS2)-dependent early colonization and inflammation kinetics faster than those of closely related S. enterica serovar Typhimurium. To investigate the accelerated TTSS-2-dependent pathogenic potential of S. Enteritidis, we focused on its genome. Results of a previously published comparative genomic study revealed the presence of mutually exclusive genes in both serovars. In this study, we investigated the roles of six S. Enteritidis-specific genes in vivo by using differential fluorescence induction (DFI) through putative gene-specific promoters. The promoter construct associated with the gene locus SEN1140 induced green fluorescent protein (GFP) expression in the gut lumen, lamina propria, mesenteric lymph nodes, and related systemic organs. To further investigate the potential role of SEN1140, we compared a SEN1140 deletion mutant with S. Typhimurium in a TTSS1-deficient background. Interestingly, the S. Enteritidis mutant lacking SEN1140 did not show the unique TTSS-2-dependent early colonization and inflammation kinetic phenotype of S. Typhimurium. Consistent with this result, complementation of SEN1140 restored the TTSS-2-dependent accelerated inflammatory potential of S. Enteritidis. This report presents a suitable screening strategy that uses a combination of DFI, fluorescence-activated cell sorting, quantitative PCR, and wild-type isogenic tagged-strain techniques to explore the unique roles of S. Enteritidis-specific genes in bacterial pathogenesis. © 2012, American Society for Microbiology.
Source Title: Infection and Immunity
URI: https://scholarbank.nus.edu.sg/handle/10635/180822
ISSN: 0019-9567
DOI: 10.1128/IAI.00180-12
Rights: Attribution 4.0 International
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