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https://doi.org/10.3892/ijo.2012.1633
Title: | The effect of Aurora kinases on cell proliferation, cell cycle regulation and metastasis in renal cell carcinoma | Authors: | Li, Y Zhou, W Wei, L Jin, J Tang, K Li, C Teh, B.T Chen, X |
Keywords: | aurora A kinase aurora B kinase aurora C kinase cyclin B cyclin dependent kinase 1 microRNA protein tyrosine phosphatase tozasertib animal model animal tissue antineoplastic activity article cell proliferation cell viability controlled study down regulation enzyme activity enzyme inhibition female G2 phase cell cycle checkpoint in vitro study in vivo study kidney carcinoma metastasis molecularly targeted therapy nonhuman priority journal protein expression tumor volume tumor xenograft upregulation Animals Carcinoma, Renal Cell Cell Cycle Cell Cycle Proteins Cell Line, Tumor Cell Proliferation Female Gene Expression Regulation, Neoplastic Humans Kidney Neoplasms Mice Mice, Nude Neoplasm Metastasis Piperazines Protein-Serine-Threonine Kinases RNA Interference Transplantation, Heterologous Tumor Burden |
Issue Date: | 2012 | Citation: | Li, Y, Zhou, W, Wei, L, Jin, J, Tang, K, Li, C, Teh, B.T, Chen, X (2012). The effect of Aurora kinases on cell proliferation, cell cycle regulation and metastasis in renal cell carcinoma. International Journal of Oncology 41 (6) : 2139-2149. ScholarBank@NUS Repository. https://doi.org/10.3892/ijo.2012.1633 | Rights: | Attribution 4.0 International | Abstract: | Aurora kinases have been shown to be involved in the regulation of the cell cycle and are related to tumor progression. This suggests the possibility that they can serve as new anticancer targets for tumor treatment. However, the important roles that Aurora kinases and their signaling pathway play in renal cell carcinoma (RCC) are not fully understood and addressed to date. In this study, we aimed to address these questions. We observed that downregulation of Aurora kinases induced by AurA miRNA, AurB miRNA or VX680 could inhibit proliferation and metastasis, induce G2/M phase arrest in clear cell renal cell carcinoma cells and exert antitumor activity in an SN12C xenograft model. We also show that either silencing of Aurora kinases or treating the cells with VX680 could downregulate the expression of cdc25c and cyclin B/cdc2, upregulate the expression of p-cdc2 (Tyr15) via blocking the activity of ERK. All these changes may contribute to inhibition of proliferation, metastasis and G2/M arrest in ccRCC. In summary, we proved that both Aurora kinases A and B are key elements of tumor growth regulation, and inhibition of Aurora kinases may contribute to blocking ccRCC progression. We conclude that Aurora kinases could be potential therapeutic targets in the management of renal cell carcinoma. | Source Title: | International Journal of Oncology | URI: | https://scholarbank.nus.edu.sg/handle/10635/180815 | ISSN: | 1019-6439 | DOI: | 10.3892/ijo.2012.1633 | Rights: | Attribution 4.0 International |
Appears in Collections: | Staff Publications Elements |
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