Please use this identifier to cite or link to this item: https://doi.org/10.1038/srep15702
Title: Choroidal neovascularization is inhibited via an intraocular decrease of inflammatory cells in mice lacking complement component C3
Authors: Tan, X
Fujiu, K
Manabe, I
Nishida, J
Yamagishi, R
Nagai, R
Yanagi, Y 
Keywords: complement component C3
animal
C57BL mouse
disease model
granulocyte
inflammation
macrophage
macular degeneration
male
metabolism
monocyte
mouse
pathology
retinal pigment epithelium
subretinal neovascularization
Animals
Choroidal Neovascularization
Complement C3
Disease Models, Animal
Granulocytes
Inflammation
Macrophages
Macular Degeneration
Male
Mice
Mice, Inbred C57BL
Monocytes
Retinal Pigment Epithelium
Issue Date: 2015
Publisher: Nature Publishing Group
Citation: Tan, X, Fujiu, K, Manabe, I, Nishida, J, Yamagishi, R, Nagai, R, Yanagi, Y (2015). Choroidal neovascularization is inhibited via an intraocular decrease of inflammatory cells in mice lacking complement component C3. Scientific Reports 5 : 15702. ScholarBank@NUS Repository. https://doi.org/10.1038/srep15702
Rights: Attribution 4.0 International
Abstract: In early age-related macular degeneration (AMD), complement component C3 can be observed in drusen, which is the accumulation of material beneath the retinal pigment epithelium. The complement pathways, via the activation of C3, can upregulate the expression of cytokines and their receptors and the recruitment of inflammatory leukocytes, both of which play an important role in the development of choroidal neovascularization (CNV) in exudative AMD. Laser-induced CNV lesions were found to be significantly smaller in C3 -/- mice than in wild-type mice. By using flow cytometry, we demonstrated that the proportions of intraocular granulocytes, CD11b + F4/80 + Ly6C hi and CD11b + F4/80 + Ly6C lo cells, were lower in C3 -/- mice than in wild-type mice as early as day 1 after laser injury, and the proportions of granulocytes and three macrophage/monocyte subsets were significantly lower on day 3. In contrast, C3 -/- mice had more granulocytes and CD11b + F4/80 + Ly6C hi cells in peripheral blood than wild-type mice after injury. Further, the expression levels of Vegfa164 were upregulated in intraocular Ly6C hi macrophages/monocytes of C3 -/- mice, but not as much as in wild-type mice. Collectively, our data demonstrate that despite a more pronounced induction of systemic inflammation, inhibition of complement factor C3 suppresses CNV by decreasing the recruitment of inflammatory cells to the lesion.
Source Title: Scientific Reports
URI: https://scholarbank.nus.edu.sg/handle/10635/180420
ISSN: 2045-2322
DOI: 10.1038/srep15702
Rights: Attribution 4.0 International
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