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Title: Identification and validation of multiple cell surface markers of clinical-grade adipose-derived mesenchymal stromal cells as novel release criteria for good manufacturing practice-compliant production
Authors: Camilleri, E.T
Gustafson, M.P
Dudakovic, A
Riester, S.M
Garces, C.G
Paradise, C.R
Takai, H
Karperien, M
Cool, S 
Sampen, H.-J.I
Larson, A.N
Qu, W
Smith, J
Dietz, A.B
Van Wijnen, A.J
Keywords: 5' nucleotidase
CD14 antigen
CD146 antigen
CD163 antigen
CD200 antigen
CD276 antibody
CD34 antibody
CD36 antigen
CD45 antigen
Hermes antigen
HLA antigen
HLA DR antigen
neurotrophin receptor p75
platelet derived growth factor beta receptor
programmed death 1 ligand 1
programmed death 1 ligand 2
Thy 1 antigen
unclassified drug
biological marker
adipose derived mesenchymal stromal cell
adipose tissue cell
cell lysate
controlled study
flow cytometry
gene expression
high throughput screening
human cell
mesenchymal stroma cell
priority journal
quantitative analysis
real time polymerase chain reaction
reverse transcription polymerase chain reaction
RNA sequence
adipose tissue
bone marrow cell
cell culture
cell proliferation
mesenchymal stroma cell
sequence analysis
Adipose Tissue
Bone Marrow Cells
Cell Proliferation
Cells, Cultured
Flow Cytometry
Mesenchymal Stromal Cells
Sequence Analysis, RNA
Issue Date: 2016
Citation: Camilleri, E.T, Gustafson, M.P, Dudakovic, A, Riester, S.M, Garces, C.G, Paradise, C.R, Takai, H, Karperien, M, Cool, S, Sampen, H.-J.I, Larson, A.N, Qu, W, Smith, J, Dietz, A.B, Van Wijnen, A.J (2016). Identification and validation of multiple cell surface markers of clinical-grade adipose-derived mesenchymal stromal cells as novel release criteria for good manufacturing practice-compliant production. Stem Cell Research and Therapy 7 (1) : 1-16. ScholarBank@NUS Repository.
Rights: Attribution 4.0 International
Abstract: Background: Clinical translation of mesenchymal stromal cells (MSCs) necessitates basic characterization of the cell product since variability in biological source and processing of MSCs may impact therapeutic outcomes. Although expression of classical cell surface markers (e.g., CD90, CD73, CD105, and CD44) is used to define MSCs, identification of functionally relevant cell surface markers would provide more robust release criteria and options for quality control. In addition, cell surface expression may distinguish between MSCs from different sources, including bone marrow-derived MSCs and clinical-grade adipose-derived MSCs (AMSCs) grown in human platelet lysate (hPL). Methods: In this work we utilized quantitative PCR, flow cytometry, and RNA-sequencing to characterize AMSCs grown in hPL and validated non-classical markers in 15 clinical-grade donors. Results: We characterized the surface marker transcriptome of AMSCs, validated the expression of classical markers, and identified nine non-classical markers (i.e., CD36, CD163, CD271, CD200, CD273, CD274, CD146, CD248, and CD140B) that may potentially discriminate AMSCs from other cell types. More importantly, these markers exhibit variability in cell surface expression among different cell isolates from a diverse cohort of donors, including freshly prepared, previously frozen, or proliferative state AMSCs and may be informative when manufacturing cells. Conclusions: Our study establishes that clinical-grade AMSCs expanded in hPL represent a homogeneous cell culture population according to classical markers,. Additionally, we validated new biomarkers for further AMSC characterization that may provide novel information guiding the development of new release criteria. © 2016 The Author(s).
Source Title: Stem Cell Research and Therapy
ISSN: 17576512
DOI: 10.1186/s13287-016-0370-8
Rights: Attribution 4.0 International
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