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https://doi.org/10.1186/s13287-016-0370-8
Title: | Identification and validation of multiple cell surface markers of clinical-grade adipose-derived mesenchymal stromal cells as novel release criteria for good manufacturing practice-compliant production | Authors: | Camilleri, E.T Gustafson, M.P Dudakovic, A Riester, S.M Garces, C.G Paradise, C.R Takai, H Karperien, M Cool, S Sampen, H.-J.I Larson, A.N Qu, W Smith, J Dietz, A.B Van Wijnen, A.J |
Keywords: | 5' nucleotidase antibody CD14 antigen CD146 antigen CD163 antigen CD200 antigen CD276 antibody CD34 antibody CD36 antigen CD45 antigen endoglin endosialin Hermes antigen HLA antigen HLA DR antigen neurotrophin receptor p75 platelet derived growth factor beta receptor programmed death 1 ligand 1 programmed death 1 ligand 2 Thy 1 antigen unclassified drug biological marker transcriptome adipose derived mesenchymal stromal cell adipose tissue cell Article cell lysate controlled study flow cytometry gene expression high throughput screening human human cell mesenchymal stroma cell priority journal quantitative analysis real time polymerase chain reaction reverse transcription polymerase chain reaction RNA sequence thrombocyte adipose tissue bone marrow cell cell culture cell proliferation cytology mesenchymal stroma cell metabolism obesity physiology procedures sequence analysis Adipose Tissue Adiposity Biomarkers Bone Marrow Cells Cell Proliferation Cells, Cultured Flow Cytometry Humans Mesenchymal Stromal Cells Sequence Analysis, RNA Transcriptome |
Issue Date: | 2016 | Citation: | Camilleri, E.T, Gustafson, M.P, Dudakovic, A, Riester, S.M, Garces, C.G, Paradise, C.R, Takai, H, Karperien, M, Cool, S, Sampen, H.-J.I, Larson, A.N, Qu, W, Smith, J, Dietz, A.B, Van Wijnen, A.J (2016). Identification and validation of multiple cell surface markers of clinical-grade adipose-derived mesenchymal stromal cells as novel release criteria for good manufacturing practice-compliant production. Stem Cell Research and Therapy 7 (1) : 1-16. ScholarBank@NUS Repository. https://doi.org/10.1186/s13287-016-0370-8 | Rights: | Attribution 4.0 International | Abstract: | Background: Clinical translation of mesenchymal stromal cells (MSCs) necessitates basic characterization of the cell product since variability in biological source and processing of MSCs may impact therapeutic outcomes. Although expression of classical cell surface markers (e.g., CD90, CD73, CD105, and CD44) is used to define MSCs, identification of functionally relevant cell surface markers would provide more robust release criteria and options for quality control. In addition, cell surface expression may distinguish between MSCs from different sources, including bone marrow-derived MSCs and clinical-grade adipose-derived MSCs (AMSCs) grown in human platelet lysate (hPL). Methods: In this work we utilized quantitative PCR, flow cytometry, and RNA-sequencing to characterize AMSCs grown in hPL and validated non-classical markers in 15 clinical-grade donors. Results: We characterized the surface marker transcriptome of AMSCs, validated the expression of classical markers, and identified nine non-classical markers (i.e., CD36, CD163, CD271, CD200, CD273, CD274, CD146, CD248, and CD140B) that may potentially discriminate AMSCs from other cell types. More importantly, these markers exhibit variability in cell surface expression among different cell isolates from a diverse cohort of donors, including freshly prepared, previously frozen, or proliferative state AMSCs and may be informative when manufacturing cells. Conclusions: Our study establishes that clinical-grade AMSCs expanded in hPL represent a homogeneous cell culture population according to classical markers,. Additionally, we validated new biomarkers for further AMSC characterization that may provide novel information guiding the development of new release criteria. © 2016 The Author(s). | Source Title: | Stem Cell Research and Therapy | URI: | https://scholarbank.nus.edu.sg/handle/10635/179959 | ISSN: | 17576512 | DOI: | 10.1186/s13287-016-0370-8 | Rights: | Attribution 4.0 International |
Appears in Collections: | Staff Publications Elements |
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