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Title: Lipidomic Profiling of Lung Pleural Effusion Identifies Unique Metabotype for EGFR Mutants in Non-Small Cell Lung Cancer
Authors: Ho, Y.S
Yip, L.Y
Basri, N
Chong, V.S.H
Teo, C.C
Tan, E
Lim, K.L 
Tan, G.S
Yang, X
Yeo, S.Y
Koh, M.S.Y 
Devanand, A 
Takano, A 
Tan, E.H
Tan, D.S.W
Lim, T.K.H 
Keywords: biological marker
EGFR protein, human
epidermal growth factor receptor
mutant protein
liquid chromatography
mass spectrometry
middle aged
non small cell lung cancer
pleura effusion
very elderly
Aged, 80 and over
Carcinoma, Non-Small-Cell Lung
Chromatography, Liquid
Mass Spectrometry
Middle Aged
Mutant Proteins
Pleural Effusion
Receptor, Epidermal Growth Factor
Issue Date: 2016
Publisher: Nature Publishing Group
Citation: Ho, Y.S, Yip, L.Y, Basri, N, Chong, V.S.H, Teo, C.C, Tan, E, Lim, K.L, Tan, G.S, Yang, X, Yeo, S.Y, Koh, M.S.Y, Devanand, A, Takano, A, Tan, E.H, Tan, D.S.W, Lim, T.K.H (2016). Lipidomic Profiling of Lung Pleural Effusion Identifies Unique Metabotype for EGFR Mutants in Non-Small Cell Lung Cancer. Scientific Reports 6 : 35110. ScholarBank@NUS Repository.
Rights: Attribution 4.0 International
Abstract: Cytology and histology forms the cornerstone for the diagnosis of non-small cell lung cancer (NSCLC) but obtaining sufficient tumour cells or tissue biopsies for these tests remains a challenge. We investigate the lipidome of lung pleural effusion (PE) for unique metabolic signatures to discriminate benign versus malignant PE and EGFR versus non-EGFR malignant subgroups to identify novel diagnostic markers that is independent of tumour cell availability. Using liquid chromatography mass spectrometry, we profiled the lipidomes of the PE of 30 benign and 41 malignant cases with or without EGFR mutation. Unsupervised principal component analysis revealed distinctive differences between the lipidomes of benign and malignant PE as well as between EGFR mutants and non-EGFR mutants. Docosapentaenoic acid and Docosahexaenoic acid gave superior sensitivity and specificity for detecting NSCLC when used singly. Additionally, several 20-and 22-carbon polyunsaturated fatty acids and phospholipid species were significantly elevated in the EGFR mutants compared to non-EGFR mutants. A 7-lipid panel showed great promise in the stratification of EGFR from non-EGFR malignant PE. Our data revealed novel lipid candidate markers in the non-cellular fraction of PE that holds potential to aid the diagnosis of benign, EGFR mutation positive and negative NSCLC. © 2016 The Author(s).
Source Title: Scientific Reports
ISSN: 2045-2322
DOI: 10.1038/srep35110
Rights: Attribution 4.0 International
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