Please use this identifier to cite or link to this item: https://doi.org/10.1038/srep45396
Title: Nerve regeneration by human corneal stromal keratocytes and stromal fibroblasts
Authors: Yam, G.H.-F 
Williams, G.P
Setiawan, M
Yusoff, N.Z.B.M
Lee, X.-W
Htoon, H.M 
Zhou, L 
Fuest, M
Mehta, J.S 
Keywords: animal
cell culture
cell proliferation
chick embryo
conditioned medium
cornea
cornea cell
cytology
growth, development and aging
human
keratomileusis
nerve regeneration
neurite
pharmacology
physiology
spinal ganglion
stroma cell
Animals
Cell Proliferation
Cells, Cultured
Chick Embryo
Cornea
Corneal Keratocytes
Culture Media, Conditioned
Ganglia, Spinal
Humans
Keratomileusis, Laser In Situ
Nerve Regeneration
Neurites
Stromal Cells
Issue Date: 2017
Publisher: Nature Publishing Group
Citation: Yam, G.H.-F, Williams, G.P, Setiawan, M, Yusoff, N.Z.B.M, Lee, X.-W, Htoon, H.M, Zhou, L, Fuest, M, Mehta, J.S (2017). Nerve regeneration by human corneal stromal keratocytes and stromal fibroblasts. Scientific Reports 7 : 45396. ScholarBank@NUS Repository. https://doi.org/10.1038/srep45396
Rights: Attribution 4.0 International
Abstract: Laser refractive surgeries reshape corneal stroma to correct refractive errors, but unavoidably affect corneal nerves. Slow nerve regeneration and atypical neurite morphology cause desensitization and neuro-epitheliopathy. Following injury, surviving corneal stromal keratocytes (CSKs) are activated to stromal fibroblasts (SFs). How these two different cell types influence nerve regeneration is elusive. Our study evaluated the neuro-regulatory effects of human SFs versus CSKs derived from the same corneal stroma using an in vitro chick dorsal root ganglion model. The neurite growth was assessed by a validated concentric circle intersection count method. Serum-free conditioned media (CM) from SFs promoted neurite growth dose-dependently, compared to that from CSKs. We detected neurotrophic and pro-inflammatory factors (interleukin-8, interleukin-15, monocyte chemoattractant protein-1, eotaxin, RANTES) in SFCM by Bio-Plex Human Cytokine assay. More than 130 proteins in SFCM and 49 in CSKCM were identified by nanoLC-MS/MS. Proteins uniquely present in SFCM had reported neuro-regulatory activities and were predicted to regulate neurogenesis, focal adhesion and wound healing. Conclusively, this was the first study showing a physiological relationship between nerve growth and the metabolically active SFs versus quiescent CSKs from the same cornea source. The dose-dependent effect on neurite growth indicated that nerve regeneration could be influenced by SF density. © The Author(s) 2017.
Source Title: Scientific Reports
URI: https://scholarbank.nus.edu.sg/handle/10635/179726
ISSN: 2045-2322
DOI: 10.1038/srep45396
Rights: Attribution 4.0 International
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