Please use this identifier to cite or link to this item: https://doi.org/10.1242/dmm.028555
Title: Upregulation of distinct collagen transcripts in post-surgery scar tissue: A study of conjunctival fibrosis
Authors: Seet, L.-F 
Toh, L.Z
Chu, S.W.L
Finger, S.N
Chua, J.L.L
Wong, T.T 
Keywords: alpha smooth muscle actin
collagen type 1
collagen type 11
collagen type 11a1
collagen type 1a1
collagen type 8
collagen type 8a1
collagen type 8a2
fibril associated collagen
messenger RNA
transforming growth factor beta2
unclassified drug
collagen
messenger RNA
transcriptome
transforming growth factor beta2
antibody labeling
Article
conjunctiva disease
conjunctival fibrosis
fibroblast
fibrosis
filtering operation
human
immunoblotting
inflammatory cell
macrophage
postoperative period
priority journal
protein expression
real time polymerase chain reaction
scar tissue
upregulation
vascular endothelial cell
wound healing
animal
conjunctiva
drug effects
fibrosis
genetics
metabolism
mouse
pathology
scar
Animals
Cicatrix
Collagen
Conjunctiva
Fibroblasts
Fibrosis
Humans
Mice
RNA, Messenger
Transcriptome
Transforming Growth Factor beta2
Up-Regulation
Wound Healing
Issue Date: 2017
Citation: Seet, L.-F, Toh, L.Z, Chu, S.W.L, Finger, S.N, Chua, J.L.L, Wong, T.T (2017). Upregulation of distinct collagen transcripts in post-surgery scar tissue: A study of conjunctival fibrosis. DMM Disease Models and Mechanisms 10 (6) : 751-760. ScholarBank@NUS Repository. https://doi.org/10.1242/dmm.028555
Rights: Attribution 4.0 International
Abstract: Excessive accumulation of collagen is often used to assess the development of fibrosis. This study aims to identify collagen genes that define fibrosis in the conjunctiva following glaucoma filtration surgery (GFS). Using the mouse model of GFS, we have identified collagen transcripts that were upregulated in the fibrotic phase of wound healing via RNA-seq. The collagen transcripts that were increased the most were encoded by Col8a1, Col11a1 and Col8a2. Further analysis of the Col8a1, Col11a1 and Col8a2 transcripts revealed their increase by 67-, 54- and 18-fold, respectively, in the fibrotic phase, compared with 12-fold for Col1a1, the most commonly evaluated collagen gene for fibrosis. However, only type I collagen was significantly upregulated at the protein level in the fibrotic phase. Type VIII and type I collagens colocalized in fibrous structures and in ACTA2-positive pericytes, and appeared to compensate for each other in expression levels. Type XI collagen showed low colocalization with both type VIII and type I collagens but can be found in association with macrophages. Furthermore, we show that both mouse and human conjunctival fibroblasts expressed elevated levels of the most highly expressed collagen genes in response to TGFâ2 treatment. Importantly, conjunctival tissues from individuals whose GF surgeries have failed due to scarring showed 3.60- and 2.78-fold increases in type VIII and I collagen transcripts, respectively, compared with those from individuals with no prior surgeries. These data demonstrate that distinct collagen transcripts are expressed at high levels in the conjunctiva after surgery and their unique expression profiles may imply differential influences on the fibrotic outcome. © 2017. Published by The Company of Biologists Ltd.
Source Title: DMM Disease Models and Mechanisms
URI: https://scholarbank.nus.edu.sg/handle/10635/179492
ISSN: 17548403
DOI: 10.1242/dmm.028555
Rights: Attribution 4.0 International
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