Please use this identifier to cite or link to this item: https://doi.org/10.3389/fmicb.2017.01172
Title: Modulation of inter-kingdom communication by PhcBSR quorum sensing system in Ralstonia solanacearum phylotype I strain GMI1000
Authors: Li, P
Yin, W
Yan, J
Chen, Y
Fu, S
Song, S
Zhou, J
Lyu, M
Deng, Y
Zhang, L.-H 
Keywords: genomic DNA
lipopeptide
ralsolamycin
unclassified drug
antimicrobial activity
Article
bacterial gene
bacterial growth
cell density
chlamydospore
deletion mutant
fungus
fungus growth
Fusarium oxysporum f. sp. cubense
gene amplification
gene expression
genetic transcription
growth inhibition
inhibition zone
liquid chromatography-mass spectrometry
nonhuman
organismal interaction
quantitative analysis
quorum sensing
Ralstonia solanacearum
real time polymerase chain reaction
rmy gene
Sporisorium scitamineum
Issue Date: 2017
Citation: Li, P, Yin, W, Yan, J, Chen, Y, Fu, S, Song, S, Zhou, J, Lyu, M, Deng, Y, Zhang, L.-H (2017). Modulation of inter-kingdom communication by PhcBSR quorum sensing system in Ralstonia solanacearum phylotype I strain GMI1000. Frontiers in Microbiology 8 (JUN) : 1172. ScholarBank@NUS Repository. https://doi.org/10.3389/fmicb.2017.01172
Rights: Attribution 4.0 International
Abstract: Ralstonia solanacearum is a ubiquitous soil-borne plant pathogenic bacterium, which frequently encounters and interacts with other soil cohabitants in competition for environmental niches. Ralsolamycin, which is encoded by the rmy genes, has been characterized as a novel inter-kingdom interaction signal that induces chlamydospore development in fungi. In this study, we provide the first genetic evidence that the rmy gene expression is controlled by the PhcBSR quorum sensing (QS) system in strain GMI1000. Mutation of phcB could lead to significant reduction of the expression levels of the genes involved in ralsolamycin biosynthesis. In addition, both the phcB and rmy mutants were attenuated in induction of chlamydospore formation in Fusarium oxysporum f. cubense and diminished in the ability to compete with the sugarcane pathogen Sporisorium scitamineum. Agreeable with the pattern of QS regulation, transcriptional expression analysis showed that the transcripts of the rmy genes were increased along with the increment of the bacterial population density. Taken together, the above findings provide new insights into the regulatory mechanisms that the QS system involves in governing the ralsolamycin inter-kingdom signaling system. @ 2017 Li, Yin, Yan, Chen, Fu, Song, Zhou, Lyu, Deng and Zhang.
Source Title: Frontiers in Microbiology
URI: https://scholarbank.nus.edu.sg/handle/10635/179482
ISSN: 1664302X
DOI: 10.3389/fmicb.2017.01172
Rights: Attribution 4.0 International
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