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https://doi.org/10.15252/msb.20177754
Title: | RNA polymerase II primes Polycomb-repressed developmental genes throughout terminal neuronal differentiation | Authors: | Ferrai, C Torlai Triglia, E Risner-Janiczek, J.R Rito, T Rackham, O.J.L de Santiago, I Kukalev, A Nicodemi, M Akalin, A Li, M Ungless, M.A Pombo, A |
Keywords: | DNA hepatocyte nuclear factor 3beta neurogenin 2 octamer transcription factor 4 polycomb repressive complex 2 RNA polymerase II semaphorin 3F transcription factor Mash1 polycomb group protein RNA polymerase II transcription factor Article cell maturation chromatin developmental gene DNA methylation DNA replication dopaminergic nerve cell electrophysiology frontal cortex human inhibition kinetics mesencephalon molecular dynamics nerve cell culture nerve cell differentiation nerve cell plasticity nonhuman phenotype pluripotent stem cell priority journal promoter region protein expression animal cell differentiation cytology genetics metabolism mouse mouse embryonic stem cell sequence analysis Animals Cell Differentiation Dopaminergic Neurons Genes, Developmental Mice Mouse Embryonic Stem Cells Polycomb-Group Proteins Promoter Regions, Genetic RNA Polymerase II Sequence Analysis, RNA Transcription Factors |
Issue Date: | 2017 | Citation: | Ferrai, C, Torlai Triglia, E, Risner-Janiczek, J.R, Rito, T, Rackham, O.J.L, de Santiago, I, Kukalev, A, Nicodemi, M, Akalin, A, Li, M, Ungless, M.A, Pombo, A (2017). RNA polymerase II primes Polycomb-repressed developmental genes throughout terminal neuronal differentiation. Molecular Systems Biology 13 (10) : 946. ScholarBank@NUS Repository. https://doi.org/10.15252/msb.20177754 | Rights: | Attribution 4.0 International | Abstract: | Polycomb repression in mouse embryonic stem cells (ESCs) is tightly associated with promoter co-occupancy of RNA polymerase II (RNAPII) which is thought to prime genes for activation during early development. However, it is unknown whether RNAPII poising is a general feature of Polycomb repression, or is lost during differentiation. Here, we map the genome-wide occupancy of RNAPII and Polycomb from pluripotent ESCs to non-dividing functional dopaminergic neurons. We find that poised RNAPII complexes are ubiquitously present at Polycomb-repressed genes at all stages of neuronal differentiation. We observe both loss and acquisition of RNAPII and Polycomb at specific groups of genes reflecting their silencing or activation. Strikingly, RNAPII remains poised at transcription factor genes which are silenced in neurons through Polycomb repression, and have major roles in specifying other, non-neuronal lineages. We conclude that RNAPII poising is intrinsically associated with Polycomb repression throughout differentiation. Our work suggests that the tight interplay between RNAPII poising and Polycomb repression not only instructs promoter state transitions, but also may enable promoter plasticity in differentiated cells. @ 2017 The Authors. Published under the terms of the CC BY 4.0 license | Source Title: | Molecular Systems Biology | URI: | https://scholarbank.nus.edu.sg/handle/10635/179455 | ISSN: | 17444292 | DOI: | 10.15252/msb.20177754 | Rights: | Attribution 4.0 International |
Appears in Collections: | Elements Staff Publications |
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