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Title: A light-up probe for detection of adenosine in urine samples by a combination of an AIE molecule and an aptamer
Authors: Hu, Y
Liu, J
You, X
Wang, C 
Li, Z
Xie, W
Keywords: Body fluids
1,2-bis[4-(triethylammoniomethyl)phenyl]-1,2-diphenylethenedibromide (TPE-2N+)
Aggregation-induced emissions
Calibration curves
Fluorescent probes
Recognition element
Relative standard deviations
Issue Date: 2017
Publisher: MDPI AG
Citation: Hu, Y, Liu, J, You, X, Wang, C, Li, Z, Xie, W (2017). A light-up probe for detection of adenosine in urine samples by a combination of an AIE molecule and an aptamer. Sensors (Switzerland) 17 (10) : 2246. ScholarBank@NUS Repository.
Rights: Attribution 4.0 International
Abstract: A light-up fluorescent probe for the detection of adenosine was constructed with an AIE (aggregation-induced emission) molecule and a DNA aptamer. The AIE molecule was used as a signal generator, and the DNA aptamer was used as a recognition element for adenosine. The emission of the AIE molecule was due to its intramolecular rotation restriction induced by the aptamer upon binding of adenosine. The optimal component ratio of the probe was AIE molecule / DNA aptamer = 100 (µM/µM). The calibration curve of adenosine detection showed a linear range of 10 pM to 0.5 µM with an R2 of 0.996, and the detection limit of the probe was 10 pM. The probe exhibited a good selectivity to adenosine against its analogs (uridine, guanosine, and cytidine). The probe was used to detect adenosine in urine samples, a recovery from 86.8% to 90.0% for the spiked concentrations of adenosine (0.01, 0.05, 0.1 µM). The relative standard deviation from 1.2% to 2.0% was obtained. The intra-day and inter-day tests also showed good precisions, with measurement RSD values of 2.3% and 2.1%, respectively. © 2017 by the authors.
Source Title: Sensors (Switzerland)
ISSN: 14248220
DOI: 10.3390/s17102246
Rights: Attribution 4.0 International
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