Please use this identifier to cite or link to this item: https://doi.org/10.3390/s17102246
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dc.titleA light-up probe for detection of adenosine in urine samples by a combination of an AIE molecule and an aptamer
dc.contributor.authorHu, Y
dc.contributor.authorLiu, J
dc.contributor.authorYou, X
dc.contributor.authorWang, C
dc.contributor.authorLi, Z
dc.contributor.authorXie, W
dc.date.accessioned2020-10-22T07:35:51Z
dc.date.available2020-10-22T07:35:51Z
dc.date.issued2017
dc.identifier.citationHu, Y, Liu, J, You, X, Wang, C, Li, Z, Xie, W (2017). A light-up probe for detection of adenosine in urine samples by a combination of an AIE molecule and an aptamer. Sensors (Switzerland) 17 (10) : 2246. ScholarBank@NUS Repository. https://doi.org/10.3390/s17102246
dc.identifier.issn14248220
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/179086
dc.description.abstractA light-up fluorescent probe for the detection of adenosine was constructed with an AIE (aggregation-induced emission) molecule and a DNA aptamer. The AIE molecule was used as a signal generator, and the DNA aptamer was used as a recognition element for adenosine. The emission of the AIE molecule was due to its intramolecular rotation restriction induced by the aptamer upon binding of adenosine. The optimal component ratio of the probe was AIE molecule / DNA aptamer = 100 (µM/µM). The calibration curve of adenosine detection showed a linear range of 10 pM to 0.5 µM with an R2 of 0.996, and the detection limit of the probe was 10 pM. The probe exhibited a good selectivity to adenosine against its analogs (uridine, guanosine, and cytidine). The probe was used to detect adenosine in urine samples, a recovery from 86.8% to 90.0% for the spiked concentrations of adenosine (0.01, 0.05, 0.1 µM). The relative standard deviation from 1.2% to 2.0% was obtained. The intra-day and inter-day tests also showed good precisions, with measurement RSD values of 2.3% and 2.1%, respectively. © 2017 by the authors.
dc.publisherMDPI AG
dc.rightsAttribution 4.0 International
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.sourceUnpaywall 20201031
dc.subjectBody fluids
dc.subjectMolecules
dc.subject1,2-bis[4-(triethylammoniomethyl)phenyl]-1,2-diphenylethenedibromide (TPE-2N+)
dc.subjectAdenosine
dc.subjectAggregation-induced emissions
dc.subjectAptamers
dc.subjectCalibration curves
dc.subjectFluorescent probes
dc.subjectRecognition element
dc.subjectRelative standard deviations
dc.subjectProbes
dc.typeArticle
dc.contributor.departmentDEPT OF CHEMICAL & BIOMOLECULAR ENGG
dc.description.doi10.3390/s17102246
dc.description.sourcetitleSensors (Switzerland)
dc.description.volume17
dc.description.issue10
dc.description.page2246
dc.published.statePublished
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