Please use this identifier to cite or link to this item: https://doi.org/10.1007/s00018-018-2757-4
Title: SLC52A3 expression is activated by NF-κB p65/Rel-B and serves as a prognostic biomarker in esophageal cancer
Authors: Long, L
Pang, X.-X
Lei, F
Zhang, J.-S
Wang, W
Liao, L.-D
Xu, X.-E
He, J.-Z
Wu, J.-Y
Wu, Z.-Y
Wang, L.-D
Lin, D.-C 
Li, E.-M
Xu, L.-Y
Keywords: biological marker
carrier protein
protein variant
riboflavin
riboflavin transporter 3
SLC52A3 protein
SLC52A3a protein
SLC52A3b protein
transcription factor RelA
transcription factor RelB
tumor necrosis factor
unclassified drug
carrier protein
isoprotein
SLC52A3 protein, human
transcription factor RelA
transcription factor RelB
tumor marker
5' flanking region
Article
cancer prognosis
cell proliferation
chromatin immunoprecipitation
colony formation
controlled study
esophageal squamous cell carcinoma
gel mobility shift assay
gene expression
genetic transcription
human
human cell
human tissue
protein expression
signal transduction
survival rate
transcription initiation site
adult
aged
binding site
esophagus tumor
female
gene expression regulation
genetics
male
metabolism
middle aged
nucleotide sequence
pathology
prognosis
squamous cell carcinoma
survival analysis
tumor cell line
5' Flanking Region
Adult
Aged
Base Sequence
Binding Sites
Biomarkers, Tumor
Carcinoma, Squamous Cell
Cell Line, Tumor
Esophageal Neoplasms
Female
Gene Expression Regulation, Neoplastic
Humans
Male
Membrane Transport Proteins
Middle Aged
Prognosis
Protein Isoforms
Survival Analysis
Transcription Factor RelA
Transcription Factor RelB
Issue Date: 2018
Publisher: Birkhauser Verlag AG
Citation: Long, L, Pang, X.-X, Lei, F, Zhang, J.-S, Wang, W, Liao, L.-D, Xu, X.-E, He, J.-Z, Wu, J.-Y, Wu, Z.-Y, Wang, L.-D, Lin, D.-C, Li, E.-M, Xu, L.-Y (2018). SLC52A3 expression is activated by NF-κB p65/Rel-B and serves as a prognostic biomarker in esophageal cancer. Cellular and Molecular Life Sciences 75 (14) : 2643-2661. ScholarBank@NUS Repository. https://doi.org/10.1007/s00018-018-2757-4
Rights: Attribution 4.0 International
Abstract: The human riboflavin transporter-3 (encoded by SLC52A3) plays a prominent role in riboflavin absorption. Interestingly, abnormal expression patterns of SLC52A3 in multiple types of human cancers have been recently noted. However, the molecular mechanisms underlying its dysregulation remain unclear. In this study, we find that SLC52A3 has two transcript variants that differ in the transcriptional start site, and encode different proteins: SLC52A3a and SLC52A3b. Importantly, aberrant expressions of SLC52A3 are associated with stepwise development of esophageal squamous cell carcinoma (ESCC) as well as the survival rates of ESCC patients. Functionally, SLC52A3a, but not SLC52A3b, strongly promotes the proliferation and colony formation of ESCC cells. Furthermore, SLC52A3 5′-flanking regions contain NF-κB p65/Rel-B-binding sites, which are crucial for mediating SLC52A3 transcriptional activity in ESCC cells. Chromatin immunoprecipitation and electrophoretic mobility shift assay reveal that p65/Rel-B bind to 5′-flanking regions of SLC52A3. Accordingly, NF-κB signaling upregulates SLC52A3 transcription upon TNFα stimulation. Taken together, these results elucidate the mechanisms underlying SLC52A3 overexpression in ESCC. More importantly, our findings identify SLC52A3 as both a predictive and prognostic biomarker for this deadly cancer. © 2018, The Author(s).
Source Title: Cellular and Molecular Life Sciences
URI: https://scholarbank.nus.edu.sg/handle/10635/179032
ISSN: 1420682X
DOI: 10.1007/s00018-018-2757-4
Rights: Attribution 4.0 International
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