Please use this identifier to cite or link to this item: https://doi.org/10.1038/srep23128
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dc.titleContinuous time Bayesian networks identify Prdm1 as a negative regulator of TH17 cell differentiation in humans
dc.contributor.authorAcerbi, E
dc.contributor.authorViganò, E
dc.contributor.authorPoidinger, M
dc.contributor.authorMortellaro, A
dc.contributor.authorZelante, T
dc.contributor.authorStella, F
dc.date.accessioned2020-10-22T03:04:57Z
dc.date.available2020-10-22T03:04:57Z
dc.date.issued2016
dc.identifier.citationAcerbi, E, Viganò, E, Poidinger, M, Mortellaro, A, Zelante, T, Stella, F (2016). Continuous time Bayesian networks identify Prdm1 as a negative regulator of TH17 cell differentiation in humans. Scientific Reports 6 : 23128. ScholarBank@NUS Repository. https://doi.org/10.1038/srep23128
dc.identifier.issn20452322
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/178934
dc.description.abstractT helper 17 (TH17) cells represent a pivotal adaptive cell subset involved in multiple immune disorders in mammalian species. Deciphering the molecular interactions regulating TH17 cell differentiation is particularly critical for novel drug target discovery designed to control maladaptive inflammatory conditions. Using continuous time Bayesian networks over a time-course gene expression dataset, we inferred the global regulatory network controlling TH17 differentiation. From the network, we identified the Prdm1 gene encoding the B lymphocyte-induced maturation protein 1 as a crucial negative regulator of human TH17 cell differentiation. The results have been validated by perturbing Prdm1 expression on freshly isolated CD4 + naïve T cells: reduction of Prdm1 expression leads to augmentation of IL-17 release. These data unravel a possible novel target to control TH17 polarization in inflammatory disorders. Furthermore, this study represents the first in vitro validation of continuous time Bayesian networks as gene network reconstruction method and as hypothesis generation tool for wet-lab biological experiments.
dc.rightsAttribution 4.0 International
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.sourceUnpaywall 20201031
dc.subjectinterleukin 17
dc.subjectPRDM1 protein, human
dc.subjectrepressor protein
dc.subjectBayes theorem
dc.subjectCD4+ T lymphocyte
dc.subjectcell culture
dc.subjectcell differentiation
dc.subjectcytology
dc.subjectfetus blood
dc.subjectgene expression regulation
dc.subjectgene regulatory network
dc.subjectgenetics
dc.subjecthuman
dc.subjectmetabolism
dc.subjectTh17 cell
dc.subjectBayes Theorem
dc.subjectCD4-Positive T-Lymphocytes
dc.subjectCell Differentiation
dc.subjectCells, Cultured
dc.subjectFetal Blood
dc.subjectGene Expression Regulation
dc.subjectGene Regulatory Networks
dc.subjectHumans
dc.subjectInterleukin-17
dc.subjectRepressor Proteins
dc.subjectTh17 Cells
dc.typeArticle
dc.contributor.departmentBIOLOGY (NU)
dc.description.doi10.1038/srep23128
dc.description.sourcetitleScientific Reports
dc.description.volume6
dc.description.page23128
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