Please use this identifier to cite or link to this item: https://doi.org/10.1038/srep40737
DC FieldValue
dc.titleIndividualised multiplexed circulating tumour DNA assays for monitoring of tumour presence in patients after colorectal cancer surgery
dc.contributor.authorNg, S.B
dc.contributor.authorChua, C
dc.contributor.authorNg, M
dc.contributor.authorGan, A
dc.contributor.authorPoon, P.S
dc.contributor.authorTeo, M
dc.contributor.authorFu, C
dc.contributor.authorLeow, W.Q
dc.contributor.authorLim, K.H
dc.contributor.authorChung, A
dc.contributor.authorKoo, S.-L
dc.contributor.authorChoo, S.P
dc.contributor.authorHo, D
dc.contributor.authorRozen, S
dc.contributor.authorTan, P
dc.contributor.authorWong, M
dc.contributor.authorBurkholder, W.F
dc.contributor.authorTan, I.B
dc.date.accessioned2020-10-21T08:02:57Z
dc.date.available2020-10-21T08:02:57Z
dc.date.issued2017
dc.identifier.citationNg, S.B, Chua, C, Ng, M, Gan, A, Poon, P.S, Teo, M, Fu, C, Leow, W.Q, Lim, K.H, Chung, A, Koo, S.-L, Choo, S.P, Ho, D, Rozen, S, Tan, P, Wong, M, Burkholder, W.F, Tan, I.B (2017). Individualised multiplexed circulating tumour DNA assays for monitoring of tumour presence in patients after colorectal cancer surgery. Scientific Reports 7 : 40737. ScholarBank@NUS Repository. https://doi.org/10.1038/srep40737
dc.identifier.issn20452322
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/178707
dc.description.abstractCirculating tumour DNA (ctDNA) has the potential to be a specific biomarker for the monitoring of tumours in patients with colorectal cancer (CRC). Here, our aim was to develop a personalised surveillance strategy to monitor the clinical course of CRC after surgery. We developed patient-specific ctDNA assays based on multiplexed detection of somatic mutations identified from patient primary tumours, and applied them to detect ctDNA in 44 CRC patients, analysing a total of 260 plasma samples. We found that ctDNA detection correlated with clinical events-it is detectable in pre-operative but not post-operative plasma, and also in patients with recurrent CRC. We also detected ctDNA in 11 out of 15 cases at or before clinical or radiological recurrence of CRC, indicating the potential of our assay for early detection of metastasis. We further present data from a patient with multiple primary cancers to demonstrate the specificity of our assays to distinguish between CRC recurrence and a second primary cancer. Our approach can complement current methods for surveillance of CRC by adding an individualised biological component, allowing us not only to point to the presence of residual or recurrent disease, but also attribute it to the original cancer. © The Author(s) 2017.
dc.rightsAttribution 4.0 International
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.sourceUnpaywall 20201031
dc.subjectcirculating tumor DNA
dc.subjectDNA
dc.subjecttumor marker
dc.subjectblood
dc.subjectcolorectal tumor
dc.subjectgenetics
dc.subjecthuman
dc.subjectmultiplex polymerase chain reaction
dc.subjectmutation
dc.subjectpostoperative period
dc.subjectrecurrent disease
dc.subjectreproducibility
dc.subjectsensitivity and specificity
dc.subjecttreatment outcome
dc.subjectworkflow
dc.subjectBiomarkers, Tumor
dc.subjectCirculating Tumor DNA
dc.subjectColorectal Neoplasms
dc.subjectDNA, Neoplasm
dc.subjectHumans
dc.subjectMultiplex Polymerase Chain Reaction
dc.subjectMutation
dc.subjectPostoperative Period
dc.subjectRecurrence
dc.subjectReproducibility of Results
dc.subjectSensitivity and Specificity
dc.subjectTreatment Outcome
dc.subjectWorkflow
dc.typeArticle
dc.contributor.departmentDUKE-NUS MEDICAL SCHOOL
dc.contributor.departmentDEPT OF MEDICINE
dc.description.doi10.1038/srep40737
dc.description.sourcetitleScientific Reports
dc.description.volume7
dc.description.page40737
Appears in Collections:Elements
Staff Publications

Show simple item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
10_1038_srep40737.pdf2.7 MBAdobe PDF

OPEN

NoneView/Download

SCOPUSTM   
Citations

33
checked on Sep 23, 2021

Page view(s)

91
checked on Sep 23, 2021

Google ScholarTM

Check

Altmetric


This item is licensed under a Creative Commons License Creative Commons