Please use this identifier to cite or link to this item: https://doi.org/10.1038/srep43745
Title: Dietary restriction protects against diethylnitrosamine-induced hepatocellular tumorigenesis by restoring the disturbed gene expression profile
Authors: Duan, T
Sun, W
Zhang, M
Ge, J
He, Y
Zhang, J
Zheng, Y
Yang, W
Shen, H.-M 
Yang, J
Zhu, X
Yu, P
Keywords: diethylnitrosamine
transcriptome
animal
apoptosis
body weight
caloric restriction
cell proliferation
cell transformation
chemically induced
gene expression profiling
gene expression regulation
gene regulatory network
genetics
male
metabolism
mouse
Animals
Apoptosis
Body Weight
Caloric Restriction
Cell Proliferation
Cell Transformation, Neoplastic
Diethylnitrosamine
Gene Expression Profiling
Gene Expression Regulation
Gene Regulatory Networks
Male
Mice
Transcriptome
Issue Date: 2017
Citation: Duan, T, Sun, W, Zhang, M, Ge, J, He, Y, Zhang, J, Zheng, Y, Yang, W, Shen, H.-M, Yang, J, Zhu, X, Yu, P (2017). Dietary restriction protects against diethylnitrosamine-induced hepatocellular tumorigenesis by restoring the disturbed gene expression profile. Scientific Reports 7 : 43745. ScholarBank@NUS Repository. https://doi.org/10.1038/srep43745
Rights: Attribution 4.0 International
Abstract: Hepatocellular carcinoma (HCC) is one of the most lethal and prevalent malignancies, worse still, there are very limited therapeutic measures with poor clinical outcomes. Dietary restriction (DR) has been known to inhibit spontaneous and induced tumors in several species, but the mechanisms are little known. In the current study, by using a diethylnitrosamine (DEN)-induced HCC mice model, we found that DR significantly reduced the hepatic tumor number and size, delayed tumor development, suppressed proliferation and promoted apoptosis. Further transcriptome sequencing of liver tissues from the DEN and the DEN accompanied with DR (DEN+DR) mice showed that DEN induced profound changes in the gene expression profile, especially in cancer-related pathways while DR treatment reversed most of the disturbed gene expression induced by DEN. Finally, transcription factor enrichment analysis uncovered the transcription factor specificity protein 1 (SP1) probably functioned as the main regulator of gene changes, orchestrating the protective effects of DR on DEN induced HCC. Taken together, by the first comprehensive transcriptome analysis, we elucidate that DR protects aginst DEN-induced HCC by restoring the disturbed gene expression profile, which holds the promise to provide effective molecular targets for cancer therapies. © The Author(s) 2017.
Source Title: Scientific Reports
URI: https://scholarbank.nus.edu.sg/handle/10635/178694
ISSN: 20452322
DOI: 10.1038/srep43745
Rights: Attribution 4.0 International
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