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https://doi.org/10.1038/srep43745
Title: | Dietary restriction protects against diethylnitrosamine-induced hepatocellular tumorigenesis by restoring the disturbed gene expression profile | Authors: | Duan, T Sun, W Zhang, M Ge, J He, Y Zhang, J Zheng, Y Yang, W Shen, H.-M Yang, J Zhu, X Yu, P |
Keywords: | diethylnitrosamine transcriptome animal apoptosis body weight caloric restriction cell proliferation cell transformation chemically induced gene expression profiling gene expression regulation gene regulatory network genetics male metabolism mouse Animals Apoptosis Body Weight Caloric Restriction Cell Proliferation Cell Transformation, Neoplastic Diethylnitrosamine Gene Expression Profiling Gene Expression Regulation Gene Regulatory Networks Male Mice Transcriptome |
Issue Date: | 2017 | Citation: | Duan, T, Sun, W, Zhang, M, Ge, J, He, Y, Zhang, J, Zheng, Y, Yang, W, Shen, H.-M, Yang, J, Zhu, X, Yu, P (2017). Dietary restriction protects against diethylnitrosamine-induced hepatocellular tumorigenesis by restoring the disturbed gene expression profile. Scientific Reports 7 : 43745. ScholarBank@NUS Repository. https://doi.org/10.1038/srep43745 | Rights: | Attribution 4.0 International | Abstract: | Hepatocellular carcinoma (HCC) is one of the most lethal and prevalent malignancies, worse still, there are very limited therapeutic measures with poor clinical outcomes. Dietary restriction (DR) has been known to inhibit spontaneous and induced tumors in several species, but the mechanisms are little known. In the current study, by using a diethylnitrosamine (DEN)-induced HCC mice model, we found that DR significantly reduced the hepatic tumor number and size, delayed tumor development, suppressed proliferation and promoted apoptosis. Further transcriptome sequencing of liver tissues from the DEN and the DEN accompanied with DR (DEN+DR) mice showed that DEN induced profound changes in the gene expression profile, especially in cancer-related pathways while DR treatment reversed most of the disturbed gene expression induced by DEN. Finally, transcription factor enrichment analysis uncovered the transcription factor specificity protein 1 (SP1) probably functioned as the main regulator of gene changes, orchestrating the protective effects of DR on DEN induced HCC. Taken together, by the first comprehensive transcriptome analysis, we elucidate that DR protects aginst DEN-induced HCC by restoring the disturbed gene expression profile, which holds the promise to provide effective molecular targets for cancer therapies. © The Author(s) 2017. | Source Title: | Scientific Reports | URI: | https://scholarbank.nus.edu.sg/handle/10635/178694 | ISSN: | 20452322 | DOI: | 10.1038/srep43745 | Rights: | Attribution 4.0 International |
Appears in Collections: | Elements Staff Publications |
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