Please use this identifier to cite or link to this item:
https://doi.org/10.1038/s41598-017-09968-7
DC Field | Value | |
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dc.title | Plasma biomarker proteins for detection of human growth hormone administration in athletes | |
dc.contributor.author | Tan, S.-H | |
dc.contributor.author | Lee, A | |
dc.contributor.author | Pascovici, D | |
dc.contributor.author | Care, N | |
dc.contributor.author | Birzniece, V | |
dc.contributor.author | Ho, K | |
dc.contributor.author | Molloy, M.P | |
dc.contributor.author | Khan, A | |
dc.date.accessioned | 2020-10-20T10:26:07Z | |
dc.date.available | 2020-10-20T10:26:07Z | |
dc.date.issued | 2017 | |
dc.identifier.citation | Tan, S.-H, Lee, A, Pascovici, D, Care, N, Birzniece, V, Ho, K, Molloy, M.P, Khan, A (2017). Plasma biomarker proteins for detection of human growth hormone administration in athletes. Scientific Reports 7 (1) : 10039. ScholarBank@NUS Repository. https://doi.org/10.1038/s41598-017-09968-7 | |
dc.identifier.issn | 2045-2322 | |
dc.identifier.uri | https://scholarbank.nus.edu.sg/handle/10635/178585 | |
dc.description.abstract | Human growth hormone (GH) is a naturally occurring hormone secreted by the pituitary gland with anabolic and growth-promoting activities. Since an increased availability of recombinant GH (rGH) for the treatment of GH-deficient patients, GH has been abused in sports and it is prohibited. "GH-isoform" and "biomarkers" tests are currently available for detection of GH abuse in sports, however both methods suffer from shortcomings. Here, we report on a proteomic approach to search for novel protein biomarkers associated with rGH administration in non-elite athletes. In this study, participants received either placebo or rGH for 8 weeks, and were followed over a 6-week washout period. We used 2-D DIGE and iTRAQ LC-MS/MS analyses to expose rGH-dependent marker proteins. Eight rGH-dependent plasma proteins namely apolipoproptein-L1, alpha-HS-glycoprotein, vitamin D-binding protein, afamin, insulin-like growth factor-binding protein-3, insulin-like growth factor-binding protein-ALS, lumican and extracellular matrix proteins 1 were identified. Apolipoprotein L1 and alpha-HS-glycoprotein were validated by Western blots to confirm their identities and expression patterns in rGH- and placebo-treated subject cohorts. Independent confirmation of these putative GH-responsive biomarkers would be of value for clinical practices and may have sports anti-doping utility. © 2017 The Author(s). | |
dc.publisher | Nature Publishing Group | |
dc.rights | Attribution 4.0 International | |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
dc.source | Unpaywall 20201031 | |
dc.subject | apolipoprotein L1 | |
dc.subject | biological marker | |
dc.subject | fetuin A | |
dc.subject | human growth hormone | |
dc.subject | proteome | |
dc.subject | adolescent | |
dc.subject | adult | |
dc.subject | athlete | |
dc.subject | blood | |
dc.subject | chemistry | |
dc.subject | controlled study | |
dc.subject | female | |
dc.subject | human | |
dc.subject | male | |
dc.subject | matrix-assisted laser desorption-ionization mass spectrometry | |
dc.subject | procedures | |
dc.subject | randomized controlled trial | |
dc.subject | substance abuse | |
dc.subject | Adolescent | |
dc.subject | Adult | |
dc.subject | alpha-2-HS-Glycoprotein | |
dc.subject | Apolipoprotein L1 | |
dc.subject | Athletes | |
dc.subject | Biomarkers | |
dc.subject | Female | |
dc.subject | Human Growth Hormone | |
dc.subject | Humans | |
dc.subject | Male | |
dc.subject | Proteome | |
dc.subject | Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization | |
dc.subject | Substance Abuse Detection | |
dc.type | Article | |
dc.contributor.department | DEPT OF MEDICINE | |
dc.description.doi | 10.1038/s41598-017-09968-7 | |
dc.description.sourcetitle | Scientific Reports | |
dc.description.volume | 7 | |
dc.description.issue | 1 | |
dc.description.page | 10039 | |
dc.published.state | published | |
Appears in Collections: | Staff Publications Elements |
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