Please use this identifier to cite or link to this item: https://doi.org/10.1038/s41598-017-09968-7
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dc.titlePlasma biomarker proteins for detection of human growth hormone administration in athletes
dc.contributor.authorTan, S.-H
dc.contributor.authorLee, A
dc.contributor.authorPascovici, D
dc.contributor.authorCare, N
dc.contributor.authorBirzniece, V
dc.contributor.authorHo, K
dc.contributor.authorMolloy, M.P
dc.contributor.authorKhan, A
dc.date.accessioned2020-10-20T10:26:07Z
dc.date.available2020-10-20T10:26:07Z
dc.date.issued2017
dc.identifier.citationTan, S.-H, Lee, A, Pascovici, D, Care, N, Birzniece, V, Ho, K, Molloy, M.P, Khan, A (2017). Plasma biomarker proteins for detection of human growth hormone administration in athletes. Scientific Reports 7 (1) : 10039. ScholarBank@NUS Repository. https://doi.org/10.1038/s41598-017-09968-7
dc.identifier.issn2045-2322
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/178585
dc.description.abstractHuman growth hormone (GH) is a naturally occurring hormone secreted by the pituitary gland with anabolic and growth-promoting activities. Since an increased availability of recombinant GH (rGH) for the treatment of GH-deficient patients, GH has been abused in sports and it is prohibited. "GH-isoform" and "biomarkers" tests are currently available for detection of GH abuse in sports, however both methods suffer from shortcomings. Here, we report on a proteomic approach to search for novel protein biomarkers associated with rGH administration in non-elite athletes. In this study, participants received either placebo or rGH for 8 weeks, and were followed over a 6-week washout period. We used 2-D DIGE and iTRAQ LC-MS/MS analyses to expose rGH-dependent marker proteins. Eight rGH-dependent plasma proteins namely apolipoproptein-L1, alpha-HS-glycoprotein, vitamin D-binding protein, afamin, insulin-like growth factor-binding protein-3, insulin-like growth factor-binding protein-ALS, lumican and extracellular matrix proteins 1 were identified. Apolipoprotein L1 and alpha-HS-glycoprotein were validated by Western blots to confirm their identities and expression patterns in rGH- and placebo-treated subject cohorts. Independent confirmation of these putative GH-responsive biomarkers would be of value for clinical practices and may have sports anti-doping utility. © 2017 The Author(s).
dc.publisherNature Publishing Group
dc.rightsAttribution 4.0 International
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.sourceUnpaywall 20201031
dc.subjectapolipoprotein L1
dc.subjectbiological marker
dc.subjectfetuin A
dc.subjecthuman growth hormone
dc.subjectproteome
dc.subjectadolescent
dc.subjectadult
dc.subjectathlete
dc.subjectblood
dc.subjectchemistry
dc.subjectcontrolled study
dc.subjectfemale
dc.subjecthuman
dc.subjectmale
dc.subjectmatrix-assisted laser desorption-ionization mass spectrometry
dc.subjectprocedures
dc.subjectrandomized controlled trial
dc.subjectsubstance abuse
dc.subjectAdolescent
dc.subjectAdult
dc.subjectalpha-2-HS-Glycoprotein
dc.subjectApolipoprotein L1
dc.subjectAthletes
dc.subjectBiomarkers
dc.subjectFemale
dc.subjectHuman Growth Hormone
dc.subjectHumans
dc.subjectMale
dc.subjectProteome
dc.subjectSpectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
dc.subjectSubstance Abuse Detection
dc.typeArticle
dc.contributor.departmentDEPT OF MEDICINE
dc.description.doi10.1038/s41598-017-09968-7
dc.description.sourcetitleScientific Reports
dc.description.volume7
dc.description.issue1
dc.description.page10039
dc.published.statepublished
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