Please use this identifier to cite or link to this item: https://doi.org/10.1038/s41598-017-09968-7
Title: Plasma biomarker proteins for detection of human growth hormone administration in athletes
Authors: Tan, S.-H 
Lee, A
Pascovici, D
Care, N
Birzniece, V
Ho, K
Molloy, M.P
Khan, A
Keywords: apolipoprotein L1
biological marker
fetuin A
human growth hormone
proteome
adolescent
adult
athlete
blood
chemistry
controlled study
female
human
male
matrix-assisted laser desorption-ionization mass spectrometry
procedures
randomized controlled trial
substance abuse
Adolescent
Adult
alpha-2-HS-Glycoprotein
Apolipoprotein L1
Athletes
Biomarkers
Female
Human Growth Hormone
Humans
Male
Proteome
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Substance Abuse Detection
Issue Date: 2017
Publisher: Nature Publishing Group
Citation: Tan, S.-H, Lee, A, Pascovici, D, Care, N, Birzniece, V, Ho, K, Molloy, M.P, Khan, A (2017). Plasma biomarker proteins for detection of human growth hormone administration in athletes. Scientific Reports 7 (1) : 10039. ScholarBank@NUS Repository. https://doi.org/10.1038/s41598-017-09968-7
Rights: Attribution 4.0 International
Abstract: Human growth hormone (GH) is a naturally occurring hormone secreted by the pituitary gland with anabolic and growth-promoting activities. Since an increased availability of recombinant GH (rGH) for the treatment of GH-deficient patients, GH has been abused in sports and it is prohibited. "GH-isoform" and "biomarkers" tests are currently available for detection of GH abuse in sports, however both methods suffer from shortcomings. Here, we report on a proteomic approach to search for novel protein biomarkers associated with rGH administration in non-elite athletes. In this study, participants received either placebo or rGH for 8 weeks, and were followed over a 6-week washout period. We used 2-D DIGE and iTRAQ LC-MS/MS analyses to expose rGH-dependent marker proteins. Eight rGH-dependent plasma proteins namely apolipoproptein-L1, alpha-HS-glycoprotein, vitamin D-binding protein, afamin, insulin-like growth factor-binding protein-3, insulin-like growth factor-binding protein-ALS, lumican and extracellular matrix proteins 1 were identified. Apolipoprotein L1 and alpha-HS-glycoprotein were validated by Western blots to confirm their identities and expression patterns in rGH- and placebo-treated subject cohorts. Independent confirmation of these putative GH-responsive biomarkers would be of value for clinical practices and may have sports anti-doping utility. © 2017 The Author(s).
Source Title: Scientific Reports
URI: https://scholarbank.nus.edu.sg/handle/10635/178585
ISSN: 2045-2322
DOI: 10.1038/s41598-017-09968-7
Rights: Attribution 4.0 International
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