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https://doi.org/10.1038/s41467-017-00935-4
DC Field | Value | |
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dc.title | The telomere binding protein Pot1 maintains haematopoietic stem cell activity with age | |
dc.contributor.author | Hosokawa, K | |
dc.contributor.author | MacArthur, B.D | |
dc.contributor.author | Ikushima, Y.M | |
dc.contributor.author | Toyama, H | |
dc.contributor.author | Masuhiro, Y | |
dc.contributor.author | Hanazawa, S | |
dc.contributor.author | Suda, T | |
dc.contributor.author | Arai, F | |
dc.date.accessioned | 2020-10-20T10:22:02Z | |
dc.date.available | 2020-10-20T10:22:02Z | |
dc.date.issued | 2017 | |
dc.identifier.citation | Hosokawa, K, MacArthur, B.D, Ikushima, Y.M, Toyama, H, Masuhiro, Y, Hanazawa, S, Suda, T, Arai, F (2017). The telomere binding protein Pot1 maintains haematopoietic stem cell activity with age. Nature Communications 8 (1) : 804. ScholarBank@NUS Repository. https://doi.org/10.1038/s41467-017-00935-4 | |
dc.identifier.issn | 2041-1723 | |
dc.identifier.uri | https://scholarbank.nus.edu.sg/handle/10635/178570 | |
dc.description.abstract | Repeated cell divisions and aging impair stem cell function. However, the mechanisms by which this occurs are not fully understood. Here we show that protection of telomeres 1A (Pot1a), a component of the Shelterin complex that protects telomeres, improves haematopoietic stem cell (HSC) activity during aging. Pot1a is highly expressed in young HSCs, but declines with age. In mouse HSCs, Pot1a knockdown increases DNA damage response (DDR) and inhibits self-renewal. Conversely, Pot1a overexpression or treatment with POT1a protein prevents DDR, maintained self-renewal activity and rejuvenated aged HSCs upon ex vivo culture. Moreover, treatment of HSCs with exogenous Pot1a inhibits the production of reactive oxygen species, suggesting a non-Telomeric role for Pot1a in HSC maintenance. Consistent with these results, treatment with exogenous human POT1 protein maintains human HSC activity in culture. Collectively, these results show that Pot1a/POT1 sustains HSC activity and can be used to expand HSC numbers ex vivo. © 2017 The Author(s). | |
dc.publisher | Nature Publishing Group | |
dc.rights | Attribution 4.0 International | |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
dc.source | Unpaywall 20201031 | |
dc.subject | Pot1 protein | |
dc.subject | reactive oxygen metabolite | |
dc.subject | telomere binding protein | |
dc.subject | unclassified drug | |
dc.subject | DNA binding protein | |
dc.subject | POT1 protein, mouse | |
dc.subject | reactive oxygen metabolite | |
dc.subject | age | |
dc.subject | cells and cell components | |
dc.subject | chromosome | |
dc.subject | DNA | |
dc.subject | gene expression | |
dc.subject | protein | |
dc.subject | reactive oxygen species | |
dc.subject | animal cell | |
dc.subject | apoptosis | |
dc.subject | Article | |
dc.subject | cell activity | |
dc.subject | cell aging | |
dc.subject | cell differentiation | |
dc.subject | cell function | |
dc.subject | cell protection | |
dc.subject | cell survival | |
dc.subject | controlled study | |
dc.subject | DNA damage response | |
dc.subject | down regulation | |
dc.subject | ex vivo study | |
dc.subject | gene expression | |
dc.subject | hematopoietic stem cell | |
dc.subject | human | |
dc.subject | human cell | |
dc.subject | microarray analysis | |
dc.subject | mouse | |
dc.subject | nonhuman | |
dc.subject | oxidative phosphorylation | |
dc.subject | protein expression | |
dc.subject | protein function | |
dc.subject | rejuvenation | |
dc.subject | senescence | |
dc.subject | stem cell culture | |
dc.subject | stem cell expansion | |
dc.subject | stem cell self-renewal | |
dc.subject | animal | |
dc.subject | cell aging | |
dc.subject | cell culture | |
dc.subject | DNA damage | |
dc.subject | genetics | |
dc.subject | hematopoietic stem cell | |
dc.subject | metabolism | |
dc.subject | physiology | |
dc.subject | telomere | |
dc.subject | Animals | |
dc.subject | Cells, Cultured | |
dc.subject | Cellular Senescence | |
dc.subject | DNA Damage | |
dc.subject | DNA-Binding Proteins | |
dc.subject | Hematopoietic Stem Cells | |
dc.subject | Humans | |
dc.subject | Mice | |
dc.subject | Reactive Oxygen Species | |
dc.subject | Telomere | |
dc.type | Article | |
dc.contributor.department | DEPT OF MEDICINE | |
dc.description.doi | 10.1038/s41467-017-00935-4 | |
dc.description.sourcetitle | Nature Communications | |
dc.description.volume | 8 | |
dc.description.issue | 1 | |
dc.description.page | 804 | |
dc.published.state | published | |
Appears in Collections: | Staff Publications Elements |
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