Please use this identifier to cite or link to this item: https://doi.org/10.1038/s41598-017-02205-1
Title: Metabolomic analysis shows differential hepatic effects of T 2 and T 3 in rats after short-term feeding with high fat diet
Authors: Iannucci, L.F
Cioffi, F
Senese, R
Goglia, F
Lanni, A
Yen, P.M 
Sinha, R.A 
Keywords: diiodothyronine
liothyronine
sphingolipid
animal
autophagy
biosynthesis
drug effect
lipid diet
lipid metabolism
lipolysis
liver
male
metabolism
metabolome
metabolomics
mitochondrion
nonalcoholic fatty liver
oxidation reduction reaction
pathology
rat
Animals
Autophagy
Diet, High-Fat
Diiodothyronines
Lipid Metabolism
Lipolysis
Liver
Male
Metabolome
Metabolomics
Mitochondria
Non-alcoholic Fatty Liver Disease
Oxidation-Reduction
Rats
Sphingolipids
Triiodothyronine
Issue Date: 2017
Citation: Iannucci, L.F, Cioffi, F, Senese, R, Goglia, F, Lanni, A, Yen, P.M, Sinha, R.A (2017). Metabolomic analysis shows differential hepatic effects of T 2 and T 3 in rats after short-term feeding with high fat diet. Scientific Reports 7 (1) : 2023. ScholarBank@NUS Repository. https://doi.org/10.1038/s41598-017-02205-1
Rights: Attribution 4.0 International
Abstract: Nonalcoholic fatty liver disease (NAFLD) is a major health problem worldwide, and is often associated with lipotoxic injury, defective mitochondrial function, and insulin resistance. Thyroid hormones (THs) are important regulators of hepatic lipid metabolism. Among the THs, diiodothyronine (T2) and triiodothyronine (T3) have shown promising results in lowering hepatic fat content in various models of NAFLD. In this study, we used a targeted metabolomics approach to investigate the differential effects of T2 and T3 on the early metabolic adaptation in the livers of rats fed high fat diet (HFD), a period when hepatosteatosis is reversible. Our results showed that both T2 and T3 strongly induced autophagy and intra-hepatic acylcarnitine flux but prevented the generation of sphingolipid/ceramides in animals fed HFD. Interestingly, although both T2 and T3 decreased hepatic fat content, only T2 was able to rescue the impairment in AKT and MAPK/ERK pathways caused by HFD. In summary, we have identified and characterized the effects of T2 and T3 on hepatic metabolism during short-term exposure to HFD. These findings illuminate the common and divergent metabolic pathways by T2 and T3 that also may be important in the prevention and treatment of NAFLD. © 2017 The Author(s).
Source Title: Scientific Reports
URI: https://scholarbank.nus.edu.sg/handle/10635/178322
ISSN: 20452322
DOI: 10.1038/s41598-017-02205-1
Rights: Attribution 4.0 International
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