Please use this identifier to cite or link to this item: https://doi.org/10.1186/1742-4690-4-90
Title: Dimerisation of HIV-2 genomic RNA is linked to efficient RNA packaging, normal particle maturation and viral infectivity
Authors: L'Hernault, A
Greatorex, J.S
Crowther, R.A
Lever, A.M.L 
Keywords: genomic RNA
mutant protein
virus RNA
virus RNA
article
controlled study
dimerization
electron microscopy
gene deletion
Human immunodeficiency virus 2
in vivo study
mutational analysis
nonhuman
RNA analysis
RNA processing
RNA sequence
signal transduction
virion
virus infectivity
virus particle
virus replication
animal
cell strain COS1
Cercopithecus
conformation
human
leukemia cell line
metabolism
molecular genetics
nucleotide sequence
pathogenicity
physiology
tumor cell line
virus replication
Human immunodeficiency virus 2
Animals
Base Sequence
Cell Line, Tumor
Cercopithecus aethiops
COS Cells
Dimerization
HIV-2
Humans
Jurkat Cells
Molecular Sequence Data
Nucleic Acid Conformation
RNA, Viral
Virus Replication
Issue Date: 2007
Publisher: BMC
Citation: L'Hernault, A, Greatorex, J.S, Crowther, R.A, Lever, A.M.L (2007). Dimerisation of HIV-2 genomic RNA is linked to efficient RNA packaging, normal particle maturation and viral infectivity. Retrovirology 4 : 90. ScholarBank@NUS Repository. https://doi.org/10.1186/1742-4690-4-90
Rights: Attribution 4.0 International
Abstract: Background: Retroviruses selectively encapsidate two copies of their genomic RNA, the Gag protein binding a specific RNA motif in the 5? UTR of the genome. In human immunodeficiency virus type 2 (HIV-2), the principal packaging signal (Psi) is upstream of the major splice donor and hence is present on all the viral RNA species. Cotranslational capture of the full length genome ensures specificity. HIV-2 RNA dimerisation is thought to occur at the dimer initiation site (DIS) located in stem-loop 1 (SL-1), downstream of the main packaging determinant. However, the HIV-2 packaging signal also contains a palindromic sequence (pal) involved in dimerisation. In this study, we analysed the role of the HIV-2 packaging signal in genomic RNA dimerisation in vivo and its implication in viral replication. Results: Using a series of deletion and substitution mutants in SL-1 and the Psi region, we show that in fully infectious HIV-2, genomic RNA dimerisation is mediated by the palindrome pal. Mutation of the DIS had no effect on dimerisation or viral infectivity, while mutations in the packaging signal severely reduce both processes as well as RNA encapsidation. Electron micrographs of the Psi-deleted virions revealed a significant reduction in the proportion of mature particles and an increase in that of particles containing multiple cores. Conclusion: In addition to its role in RNA encapsidation, the HIV-2 packaging signal contains a palindromic sequence that is critical for genomic RNA dimerisation. Encapsidation of a dimeric genome seems required for the production of infectious mature particles, and provides a promising therapeutic target. © 2007 L'Hernault et al; licensee BioMed Central Ltd.
Source Title: Retrovirology
URI: https://scholarbank.nus.edu.sg/handle/10635/178239
ISSN: 1742-4690
DOI: 10.1186/1742-4690-4-90
Rights: Attribution 4.0 International
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