Please use this identifier to cite or link to this item:
https://doi.org/10.1186/1756-9966-28-59
DC Field | Value | |
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dc.title | Development of a novel small antibody that retains specificity for tumor targeting | |
dc.contributor.author | Zhen, Z.-P | |
dc.contributor.author | Zhang, J | |
dc.contributor.author | Zhang, S.-Y | |
dc.date.accessioned | 2020-10-20T08:24:21Z | |
dc.date.available | 2020-10-20T08:24:21Z | |
dc.date.issued | 2009 | |
dc.identifier.citation | Zhen, Z.-P, Zhang, J, Zhang, S.-Y (2009). Development of a novel small antibody that retains specificity for tumor targeting. Journal of Experimental and Clinical Cancer Research 28 (1) : 59. ScholarBank@NUS Repository. https://doi.org/10.1186/1756-9966-28-59 | |
dc.identifier.issn | 1756-9966 | |
dc.identifier.uri | https://scholarbank.nus.edu.sg/handle/10635/178220 | |
dc.description.abstract | Background. For the targeted therapy of solid tumor mediated by monoclonal antibody (mAb), there have different models of rebuilding small antibodies originated from native ones. Almost all natural antibody molecules have the similar structure and conformation, but those rebuilt small antibodies cannot completely keep the original traits of parental antibodies, especially the reduced specificity, which gravely influences the efficacy of small antibodies. Methods. In this study, authors developed a novel mimetic in the form of V HFRIc-10-VHCDRI-VHFR2-V LCDR3-VLFR4N-10 for a parental mAb induced with human breast cancer, and the mimetic moiety was conjugated to the C-terminal of toxicin colicin Ia. The novel fusion peptide, named protomimecin (PMN), was administered to MCF-7 breast cancer cells to demonstrate its killing competency in vitro and in vivo. Results. Compared with original antibody-colicin Ia (Fab-Ia) and single-chain antibody-colicin Ia (Sc-Ia) fusion proteins, PMN retained the targeting specificity of parental antibody and could specifically kill MCF-7 cells in vitro. By injecting intraperitoneally into BALB/c athymic mice bearing MCF-7 tumors, with reduced affinity, PMN significantly suppressed the growth of tumors compared with control mice treated by toxicin protein, Fab-Ia protein, Sc-Ia protein or by PBS (p < 0.05). Conclusion. This novel mimetic antibody retained original specificity of parental antibody, and could effectively guide killer moiety to suppress the growth of breast cancer by targeted cell death. © 2009 Zhen et al. | |
dc.publisher | BMC | |
dc.rights | Attribution 4.0 International | |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
dc.source | Unpaywall 20201031 | |
dc.subject | antineoplastic agent | |
dc.subject | immunoglobulin F(ab) fragment | |
dc.subject | monoclonal antibody | |
dc.subject | monoclonal antibody colicin Ia | |
dc.subject | protomimecin | |
dc.subject | single chain fragment variable antibody | |
dc.subject | unclassified drug | |
dc.subject | antibody | |
dc.subject | hybrid protein | |
dc.subject | animal cell | |
dc.subject | animal cell culture | |
dc.subject | animal experiment | |
dc.subject | animal model | |
dc.subject | animal tissue | |
dc.subject | antigen specificity | |
dc.subject | article | |
dc.subject | breast cancer | |
dc.subject | cancer inhibition | |
dc.subject | carboxy terminal sequence | |
dc.subject | cell strain MCF 7 | |
dc.subject | controlled study | |
dc.subject | drug cytotoxicity | |
dc.subject | drug distribution | |
dc.subject | drug protein binding | |
dc.subject | female | |
dc.subject | human | |
dc.subject | human cell | |
dc.subject | human cell culture | |
dc.subject | in vitro study | |
dc.subject | in vivo study | |
dc.subject | mouse | |
dc.subject | nonhuman | |
dc.subject | priority journal | |
dc.subject | tumor volume | |
dc.subject | animal | |
dc.subject | antibody specificity | |
dc.subject | cell line | |
dc.subject | cell survival | |
dc.subject | drug screening | |
dc.subject | gene vector | |
dc.subject | genetics | |
dc.subject | immunology | |
dc.subject | metabolism | |
dc.subject | neoplasm | |
dc.subject | nude mouse | |
dc.subject | pathology | |
dc.subject | sensitivity and specificity | |
dc.subject | Animals | |
dc.subject | Antibodies | |
dc.subject | Antibody Specificity | |
dc.subject | Cell Line | |
dc.subject | Cell Survival | |
dc.subject | Female | |
dc.subject | Genetic Vectors | |
dc.subject | Humans | |
dc.subject | Mice | |
dc.subject | Mice, Nude | |
dc.subject | Neoplasms | |
dc.subject | Recombinant Fusion Proteins | |
dc.subject | Sensitivity and Specificity | |
dc.subject | Xenograft Model Antitumor Assays | |
dc.type | Article | |
dc.contributor.department | ECONOMICS | |
dc.description.doi | 10.1186/1756-9966-28-59 | |
dc.description.sourcetitle | Journal of Experimental and Clinical Cancer Research | |
dc.description.volume | 28 | |
dc.description.issue | 1 | |
dc.description.page | 59 | |
dc.published.state | published | |
Appears in Collections: | Staff Publications Elements |
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