Please use this identifier to cite or link to this item: https://doi.org/10.1038/msb.2012.59
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dc.titleRNAi screening reveals a large signaling network controlling the Golgi apparatus in human cells
dc.contributor.authorChia, J
dc.contributor.authorGoh, G
dc.contributor.authorRacine, V
dc.contributor.authorNg, S
dc.contributor.authorKumar, P
dc.contributor.authorBard, F
dc.date.accessioned2020-10-20T08:08:27Z
dc.date.available2020-10-20T08:08:27Z
dc.date.issued2012
dc.identifier.citationChia, J, Goh, G, Racine, V, Ng, S, Kumar, P, Bard, F (2012). RNAi screening reveals a large signaling network controlling the Golgi apparatus in human cells. Molecular Systems Biology 8 : 629. ScholarBank@NUS Repository. https://doi.org/10.1038/msb.2012.59
dc.identifier.issn1744-4292
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/178155
dc.description.abstractThe Golgi apparatus has many important physiological functions, including sorting of secretory cargo and biosynthesis of complex glycans. These functions depend on the intricate and compartmentalized organization of the Golgi apparatus. To investigate the mechanisms that regulate Golgi architecture, we developed a quantitative morphological assay using three different Golgi compartment markers and quantitative image analysis, and performed a kinome- and phosphatome-wide RNAi screen in HeLa cells. Depletion of 159 signaling genes, nearly 20% of genes assayed, induced strong and varied perturbations in Golgi morphology. Using bioinformatics data, a large regulatory network could be constructed. Specific subnetworks are involved in phosphoinositides regulation, acto-myosin dynamics and mitogen activated protein kinase signaling. Most gene depletion also affected Golgi functions, in particular glycan biosynthesis, suggesting that signaling cascades can control glycosylation directly at the Golgi level. Our results provide a genetic overview of the signaling pathways that control the Golgi apparatus in human cells. © 2012 EMBO and Macmillan Publishers Limited.
dc.publisherEMBO Press
dc.rightsAttribution 4.0 International
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.sourceUnpaywall 20201031
dc.subjectactin
dc.subjectglycan
dc.subjectmitogen activated protein kinase
dc.subjectmyosin
dc.subjectphosphatidylinositide
dc.subjectsmall interfering RNA
dc.subjectlectin
dc.subjectmyosin adenosine triphosphatase
dc.subjectphosphatase
dc.subjectpolysaccharide
dc.subjectprotein kinase
dc.subjectactin myosin interaction
dc.subjectarticle
dc.subjectbioinformatics
dc.subjectcarbohydrate metabolism
dc.subjectfemale
dc.subjectgene regulatory network
dc.subjectgenetic screening
dc.subjectGolgi complex
dc.subjectHeLa cell
dc.subjecthuman
dc.subjecthuman cell
dc.subjecthuman cell culture
dc.subjectmorphology
dc.subjectphenotype
dc.subjectpriority journal
dc.subjectRNA interference
dc.subjectbiology
dc.subjectbiosynthesis
dc.subjectcell cycle
dc.subjectchemistry
dc.subjectfluorescence microscopy
dc.subjectfluorescent antibody technique
dc.subjectgene expression regulation
dc.subjectgenetics
dc.subjectglycosylation
dc.subjectimage processing
dc.subjectmetabolism
dc.subjectpilot study
dc.subjectreproducibility
dc.subjectsignal transduction
dc.subjectActomyosin
dc.subjectCell Cycle
dc.subjectComputational Biology
dc.subjectFluorescent Antibody Technique
dc.subjectGene Expression Regulation
dc.subjectGlycosylation
dc.subjectGolgi Apparatus
dc.subjectHeLa Cells
dc.subjectHumans
dc.subjectImage Processing, Computer-Assisted
dc.subjectLectins
dc.subjectMicroscopy, Fluorescence
dc.subjectPhenotype
dc.subjectPhosphoric Monoester Hydrolases
dc.subjectPilot Projects
dc.subjectPolysaccharides
dc.subjectProtein Kinases
dc.subjectReproducibility of Results
dc.subjectRNA Interference
dc.subjectSignal Transduction
dc.typeArticle
dc.contributor.departmentDEPT OF BIOCHEMISTRY
dc.description.doi10.1038/msb.2012.59
dc.description.sourcetitleMolecular Systems Biology
dc.description.volume8
dc.description.page629
dc.published.statepublished
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