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https://doi.org/10.1038/s41598-017-19063-6
Title: | Prime-boost vaccination with recombinant protein and adenovirus-vector expressing Plasmodium vivax circumsporozoite protein (CSP) partially protects mice against Pb/Pv sporozoite challenge | Authors: | De Camargo, T.M De Freitas, E.O Gimenez, A.M Lima, L.C De Almeida Caramico, K Françoso, K.S Bruna-Romero, O Andolina, C Nosten, F Rénia, L Ertl, H.C.J Nussenzweig, R.S Nussenzweig, V Rodrigues, M.M Reyes-Sandoval, A Soares, I.S |
Keywords: | circumsporozoite protein, Protozoan immunoglobulin G malaria vaccine protozoal protein protozoon antibody recombinant protein Adenoviridae administration and dosage amino acid sequence animal antibody affinity blood chemistry disease model female gene vector genetics immunization immunology mortality mouse Plasmodium vivax Plasmodium vivax malaria vaccine immunogenicity Adenoviridae Amino Acid Sequence Animals Antibodies, Protozoan Antibody Affinity Disease Models, Animal Female Genetic Vectors Immunization Immunogenicity, Vaccine Immunoglobulin G Malaria Vaccines Malaria, Vivax Mice Plasmodium vivax Protozoan Proteins Recombinant Proteins |
Issue Date: | 2018 | Citation: | De Camargo, T.M, De Freitas, E.O, Gimenez, A.M, Lima, L.C, De Almeida Caramico, K, Françoso, K.S, Bruna-Romero, O, Andolina, C, Nosten, F, Rénia, L, Ertl, H.C.J, Nussenzweig, R.S, Nussenzweig, V, Rodrigues, M.M, Reyes-Sandoval, A, Soares, I.S (2018). Prime-boost vaccination with recombinant protein and adenovirus-vector expressing Plasmodium vivax circumsporozoite protein (CSP) partially protects mice against Pb/Pv sporozoite challenge. Scientific Reports 8 (1) : 1118. ScholarBank@NUS Repository. https://doi.org/10.1038/s41598-017-19063-6 | Rights: | Attribution 4.0 International | Abstract: | Vaccine development against Plasmodium vivax malaria lags behind that for Plasmodium falciparum. To narrow this gap, we administered recombinant antigens based on P. vivax circumsporozoite protein (CSP) to mice. We expressed in Pichia pastoris two chimeric proteins by merging the three central repeat regions of different CSP alleles (VK210, VK247, and P. vivax-like). The first construct (yPvCSP-AllFL) contained the fused repeat regions flanked by N- and C-terminal regions. The second construct (yPvCSP-AllCT) contained the fused repeat regions and the C-terminal domain, plus RI region. Mice were vaccinated with three doses of yPvCSP in adjuvants Poly (I:C) or Montanide ISA720. We also used replication-defective adenovirus vectors expressing CSP of human serotype 5 (AdHu5) and chimpanzee serotype 68 (AdC68) for priming mice which were subsequently boosted twice with yPvCSP proteins in Poly (I:C) adjuvant. Regardless of the regime used, immunized mice generated high IgG titres specific to all CSP alleles. After challenge with P. berghei ANKA transgenic parasites expressing Pb/PvVK210 or Pb/PvVK247 sporozoites, significant time delays for parasitemia were observed in all vaccinated mice. These vaccine formulations should be clinically tried for their potential as protective universal vaccine against P. vivax malaria. © 2018 The Author(s). | Source Title: | Scientific Reports | URI: | https://scholarbank.nus.edu.sg/handle/10635/177831 | ISSN: | 20452322 | DOI: | 10.1038/s41598-017-19063-6 | Rights: | Attribution 4.0 International |
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