Please use this identifier to cite or link to this item: https://doi.org/10.1039/c4nr03643c
Title: Toehold-mediated internal control to probe the near-field interaction between the metallic nanoparticle and the fluorophore
Authors: Ang, Y.S 
Yung, L.Y.L 
Keywords: Internal controls
Metallic nanoparticles
Near field interactions
carbocyanine
colloid
cyanine dye 3
DNA
fluorescent dye
gold
metal nanoparticle
oligonucleotide
thiol derivative
chemistry
colloid
kinetics
nanotechnology
particle size
procedures
spectrofluorometry
Carbocyanines
Colloids
DNA
Fluorescent Dyes
Gold
Kinetics
Metal Nanoparticles
Nanotechnology
Oligonucleotides
Particle Size
Spectrometry, Fluorescence
Sulfhydryl Compounds
Issue Date: 2014
Publisher: Royal Society of Chemistry
Citation: Ang, Y.S, Yung, L.Y.L (2014). Toehold-mediated internal control to probe the near-field interaction between the metallic nanoparticle and the fluorophore. Nanoscale 6 (21) : 12515-12523. ScholarBank@NUS Repository. https://doi.org/10.1039/c4nr03643c
Rights: Attribution 4.0 International
Abstract: Metallic nanoparticles (MNPs) are known to alter the emission of vicinal fluorophores through the near-field interaction, leading to either fluorescence quenching or enhancement. Much ambiguity remains in the experimental outcome of such a near-field interaction, particularly for bulk colloidal solution. It is hypothesized that the strong far-field interference from the inner filter effect of the MNPs could mask the true near-field MNP-fluorophore interaction significantly. Thus, in this work, a reliable internal control capable of decoupling the near-field interaction from far-field interference is established by the use of the DNA toehold concept to mediate the in situ assembly and disassembly of the MNP-fluorophore conjugate. A model gold nanoparticle (AuNP)-Cy3 system is used to investigate our proposed toehold-mediated internal control system. The maximum fluorescence enhancement is obtained for large-sized AuNP (58 nm) separated from Cy3 at an intermediate distance of 6.8 nm, while fluorescence quenching is observed for smaller-sized AuNP (11 nm and 23 nm), which is in agreement with the theoretical values reported in the literature. This work shows that the toehold-mediated internal control design can serve as a central system for evaluating the near-field interaction of other MNP-fluorophore combinations and facilitate the rational design of specific MNP-fluorophore systems for various applications. © 2014 the Partner Organisations.
Source Title: Nanoscale
URI: https://scholarbank.nus.edu.sg/handle/10635/177775
ISSN: 20403364
DOI: 10.1039/c4nr03643c
Rights: Attribution 4.0 International
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