Please use this identifier to cite or link to this item: https://doi.org/10.2337/db17-0526
Title: Adipocyte long-Noncoding RNA transcriptome analysis of obese mice identified lnc-Leptin, which regulates leptin
Authors: Lo K.A.
Huang S. 
Walet A.C.E. 
Zhang Z.-C.
Leow M.K.-S. 
Liu M.
Sun L. 
Issue Date: 2018
Publisher: American Diabetes Association Inc.
Citation: Lo K.A., Huang S., Walet A.C.E., Zhang Z.-C., Leow M.K.-S., Liu M., Sun L. (2018). Adipocyte long-Noncoding RNA transcriptome analysis of obese mice identified lnc-Leptin, which regulates leptin. Diabetes 67 (6) : 1045 - 1056. ScholarBank@NUS Repository. https://doi.org/10.2337/db17-0526
Abstract: Obesity induces profound transcriptome changes in adipocytes, and recent evidence suggests that long-noncoding RNAs (lncRNAs) play key roles in this process. We performed a comprehensive transcriptome study by RNA sequencing in adipocytes isolated from interscapular brown, inguinal, and epididymal white adipose tissue in diet-induced obese mice. The analysis revealed a set of obesity-dysregulated lncRNAs, many of which exhibit dynamic changes in the fed versus fasted state, potentially serving as novel molecular markers of adipose energy status. Among the most prominent lncRNAs is Lnc-leptin, which is transcribed from an enhancer region upstream of leptin (Lep). Expression of Lnc-leptin is sensitive to insulin and closely correlates to Lep expression across diverse pathophysiological conditions. Functionally, induction of Lnc-leptin is essential for adipogenesis, and its presence is required for the maintenance of Lep expression in vitro and in vivo. Direct interaction was detected between DNA loci of Lnc-leptin and Lep in mature adipocytes, which diminished upon Lnc-leptin knockdown. Our study establishes Lnc-leptin as a new regulator of Lep.
Source Title: Diabetes
URI: https://scholarbank.nus.edu.sg/handle/10635/177487
ISSN: 0012-1797
DOI: 10.2337/db17-0526
Appears in Collections:Staff Publications
Elements

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

28
checked on Oct 18, 2021

Page view(s)

99
checked on Oct 14, 2021

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.