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Title: Development of biosensor and electrochemical studies of carbon-based materials
Keywords: diamond, biocompatibility, surface functionalization, biosensor, graphene, electrochemical
Issue Date: 14-Oct-2009
Citation: CHONG KWOK FENG (2009-10-14). Development of biosensor and electrochemical studies of carbon-based materials. ScholarBank@NUS Repository.
Abstract: Diamond is well known for girl¿s best friend and also receives intense research interest for its superb chemical stability, biocompatibility and excellent electrochemical properties. In this research work, biocompatibility of diamond was investigated with a view towards correlating surface chemistry and topography with cellular adhesion and growth. A series of surface functionalization were performed on diamond surface and atomic force microscopy method revealed that cell adhesion forces on diamond were increased after surface functionalization. A direct correlation between initial cell adhesion forces and the subsequent cell growth was observed. With the surface functional groups, neuronal cell gradient was constructed on the diamond surface by capillary injection method. Biosensing properties of diamond were investigated by constructing whole cell biosensor on diamond electrode. Different biological entities were used, namely Chlorella vulgaris and Acidithiobacillus ferrooxidans. Whole cell biosensors were used for heavy metal ions sensing and diamond electrode was proven to resist biofouling in the sensing process. Graphene, a rapid rising star among material science and condensed physics was also studied in this research work. Electrochemical properties of grahene were studied in the aspect of background current, heterogeneous electron transfer rate constant, charge transfer resistance and activation enthalpy. It was found that the extremely low background current of graphene opens up possibility to achieve ultrasensitive biosensor on carbon-based platform.
Appears in Collections:Ph.D Theses (Open)

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