Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/176933
Title: THE ROLE OF NK CELL METABOLISM IN NK CELL ACTIVATION AND FUNCTION
Authors: WANG ZIXI
Keywords: metabolism, glycolysis, OXPHOS, natural killer cell, natural killer cell receptor, NK cell function
Issue Date: 15-Jan-2020
Citation: WANG ZIXI (2020-01-15). THE ROLE OF NK CELL METABOLISM IN NK CELL ACTIVATION AND FUNCTION. ScholarBank@NUS Repository.
Abstract: Natural killer (NK) cell activation is modulated by cytokines and NK cell receptors (NKRs). It has been found that NK cells stimulated by activating cytokines (e.g. interleukin-15) increase glycolysis and oxidative phosphorylation (OXPHOS) to support their effector functions. However, little is known about the metabolic reprogramming of human NK cells upon NKR activation. In this study, we investigated the metabolic changes of NK cells activated by NKRs. We found that NK cells upregulated glycolysis and OXPHOS in response to anti-CD16 antibody and NKG2D ligand engagement. We further investigated the impact of glycolysis and OXPHOS on NK cell effector functions. We found that inhibition of either glycolysis or OXPHOS impaired NK cell production of interferon-γ. Interestingly, inhibiting glycolysis but not OXPHOS decreased the cytotoxicity of expanded NK cells by supressing degranulation and Fas ligand expression. Thus, our study provides insight into understanding the metabolic requirements of NKR-activated human NK cells.
URI: https://scholarbank.nus.edu.sg/handle/10635/176933
Appears in Collections:Ph.D Theses (Open)

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
WangZX.pdf3.23 MBAdobe PDF

OPEN

NoneView/Download

Page view(s)

11
checked on Dec 3, 2020

Download(s)

1
checked on Dec 3, 2020

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.