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https://scholarbank.nus.edu.sg/handle/10635/17624
DC Field | Value | |
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dc.title | CD8 T cell mediated induction of interleukin-12p70 production by dendritic cells | |
dc.contributor.author | WONG KOK LOON | |
dc.date.accessioned | 2010-07-13T18:02:04Z | |
dc.date.available | 2010-07-13T18:02:04Z | |
dc.date.issued | 2009-01-21 | |
dc.identifier.citation | WONG KOK LOON (2009-01-21). CD8 T cell mediated induction of interleukin-12p70 production by dendritic cells. ScholarBank@NUS Repository. | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/17624 | |
dc.description.abstract | In this study, we set up an in vitro system to study the effects of CD8 T cells on splenic DC IL-12p70 production during an antigen specific interaction. We find that mouse splenic DCs respond to CD8 T cells with IL-12p70 production only when DCs were concurrently stimulated with TLR ligands. Different TLR ligands cooperated with CD8 T cells for IL-12p70 production to different extents. In particular, CpG was most effective for the induction of IL-12p70, while other TLR ligands, like LPS, were only moderately effective. We also found the immune status of the CD8 T cells was important for priming DCs for IL-12p70 production. Pre-activation of CD8 T cells was required for effective DC IL-12p70 priming with LPS. With CpG, naturally occurring memory CD8 T cells were superior to naC/ve CD8 T cells for the induction of IL-12p70. Furthermore, we also show that apart from IFN-N3, CD8 T cells also use CD40L, GM-CSF and TNF-N1 to prime DCs for IL-12p70 production. Of the splenic DC subsets, CD8N1+ DCs were the primary source of IL-12p70, demonstrating the specialized role of the CD8N1+ DC subset in this process. Interestingly, CD8N1+ DCs are also implicated as the principle DC subset for cross-presentation and cross-priming cytotoxic CD8 T cell responses. Our study describes a novel pathway for the induction effective IL-12p70 responses to cross-presented antigens. | |
dc.language.iso | en | |
dc.subject | immune regulation, CD8 T cells, dendritic cells | |
dc.type | Thesis | |
dc.contributor.department | NUS GRAD SCH FOR INTEGRATIVE SCI & ENGG | |
dc.contributor.supervisor | KEMENY, DAVID MICHAEL | |
dc.description.degree | Ph.D | |
dc.description.degreeconferred | DOCTOR OF PHILOSOPHY | |
dc.identifier.isiut | NOT_IN_WOS | |
Appears in Collections: | Ph.D Theses (Open) |
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wongkoklonn_thesis_final_revised.pdf | 1.95 MB | Adobe PDF | OPEN | None | View/Download |
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