Please use this identifier to cite or link to this item:
https://doi.org/10.1117/1.NPh.2.2.021013
DC Field | Value | |
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dc.title | All-optical mapping of barrel cortex circuits based on simultaneous voltage-sensitive dye imaging and channelrhodopsin-mediated photostimulation | |
dc.contributor.author | Lo, S.Q | |
dc.contributor.author | Koh, D.X.P | |
dc.contributor.author | Sng, J.C.G | |
dc.contributor.author | Augustine, G.J | |
dc.date.accessioned | 2020-09-14T08:17:17Z | |
dc.date.available | 2020-09-14T08:17:17Z | |
dc.date.issued | 2015 | |
dc.identifier.citation | Lo, S.Q, Koh, D.X.P, Sng, J.C.G, Augustine, G.J (2015). All-optical mapping of barrel cortex circuits based on simultaneous voltage-sensitive dye imaging and channelrhodopsin-mediated photostimulation. Neurophotonics 2 (2) : 14090SSR. ScholarBank@NUS Repository. https://doi.org/10.1117/1.NPh.2.2.021013 | |
dc.identifier.issn | 2329-423X | |
dc.identifier.uri | https://scholarbank.nus.edu.sg/handle/10635/176148 | |
dc.description.abstract | We describe an experimental approach that uses light to both control and detect neuronal activity in mouse barrel cortex slices: blue light patterned by a digital micromirror array system allowed us to photostimulate specific layers and columns, while a red-shifted voltage-sensitive dye was used to map out large-scale circuit activity. We demonstrate that such all-optical mapping can interrogate various circuits in somatosensory cortex by sequentially activating different layers and columns. Further, mapping in slices from whisker-deprived mice demonstrated that chronic sensory deprivation did not significantly alter feedforward inhibition driven by layer 5 pyramidal neurons. Further development of voltage-sensitive optical probes should allow this all-optical mapping approach to become an important and high-throughput tool for mapping circuit interactions in the brain. © The Authors. | |
dc.source | Unpaywall 20200831 | |
dc.subject | Brain | |
dc.subject | Mammals | |
dc.subject | Mapping | |
dc.subject | Neurons | |
dc.subject | Timing circuits | |
dc.subject | Barrel cortex | |
dc.subject | channelrhodopsin | |
dc.subject | Digital micromirror arrays | |
dc.subject | Experimental approaches | |
dc.subject | Feedforward inhibition | |
dc.subject | Optogenetics | |
dc.subject | Somatosensory cortex | |
dc.subject | Voltage-sensitive dye imaging | |
dc.subject | Brain mapping | |
dc.subject | channelrhodopsin 2 | |
dc.subject | rhodopsin | |
dc.subject | unclassified drug | |
dc.subject | animal experiment | |
dc.subject | animal tissue | |
dc.subject | Article | |
dc.subject | brain region | |
dc.subject | camera | |
dc.subject | connectome | |
dc.subject | controlled study | |
dc.subject | light emitting diode | |
dc.subject | micromirror array system | |
dc.subject | mouse | |
dc.subject | nerve cell network | |
dc.subject | nerve stimulator | |
dc.subject | neurobiology | |
dc.subject | nonhuman | |
dc.subject | optogenetics | |
dc.subject | photostimulation | |
dc.subject | protein expression | |
dc.subject | pyramidal nerve cell | |
dc.subject | sensory deprivation | |
dc.subject | somatosensory cortex | |
dc.subject | synaptic transmission | |
dc.subject | vibrissa | |
dc.subject | voltage sensitive dye imaging | |
dc.type | Article | |
dc.contributor.department | DEPT OF PHARMACOLOGY | |
dc.contributor.department | DEPT OF PHYSIOLOGY | |
dc.contributor.department | DUKE-NUS MEDICAL SCHOOL | |
dc.description.doi | 10.1117/1.NPh.2.2.021013 | |
dc.description.sourcetitle | Neurophotonics | |
dc.description.volume | 2 | |
dc.description.issue | 2 | |
dc.description.page | 14090SSR | |
dc.published.state | Published | |
Appears in Collections: | Elements Staff Publications |
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