Please use this identifier to cite or link to this item: https://doi.org/10.1117/1.NPh.2.2.021013
Title: All-optical mapping of barrel cortex circuits based on simultaneous voltage-sensitive dye imaging and channelrhodopsin-mediated photostimulation
Authors: Lo, S.Q 
Koh, D.X.P
Sng, J.C.G 
Augustine, G.J 
Keywords: Brain
Mammals
Mapping
Neurons
Timing circuits
Barrel cortex
channelrhodopsin
Digital micromirror arrays
Experimental approaches
Feedforward inhibition
Optogenetics
Somatosensory cortex
Voltage-sensitive dye imaging
Brain mapping
channelrhodopsin 2
rhodopsin
unclassified drug
animal experiment
animal tissue
Article
brain region
camera
connectome
controlled study
light emitting diode
micromirror array system
mouse
nerve cell network
nerve stimulator
neurobiology
nonhuman
optogenetics
photostimulation
protein expression
pyramidal nerve cell
sensory deprivation
somatosensory cortex
synaptic transmission
vibrissa
voltage sensitive dye imaging
Issue Date: 2015
Citation: Lo, S.Q, Koh, D.X.P, Sng, J.C.G, Augustine, G.J (2015). All-optical mapping of barrel cortex circuits based on simultaneous voltage-sensitive dye imaging and channelrhodopsin-mediated photostimulation. Neurophotonics 2 (2) : 14090SSR. ScholarBank@NUS Repository. https://doi.org/10.1117/1.NPh.2.2.021013
Abstract: We describe an experimental approach that uses light to both control and detect neuronal activity in mouse barrel cortex slices: blue light patterned by a digital micromirror array system allowed us to photostimulate specific layers and columns, while a red-shifted voltage-sensitive dye was used to map out large-scale circuit activity. We demonstrate that such all-optical mapping can interrogate various circuits in somatosensory cortex by sequentially activating different layers and columns. Further, mapping in slices from whisker-deprived mice demonstrated that chronic sensory deprivation did not significantly alter feedforward inhibition driven by layer 5 pyramidal neurons. Further development of voltage-sensitive optical probes should allow this all-optical mapping approach to become an important and high-throughput tool for mapping circuit interactions in the brain. © The Authors.
Source Title: Neurophotonics
URI: https://scholarbank.nus.edu.sg/handle/10635/176148
ISSN: 2329-423X
DOI: 10.1117/1.NPh.2.2.021013
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