Please use this identifier to cite or link to this item:
https://doi.org/10.1128/mSphereDirect.00120-18
DC Field | Value | |
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dc.title | Zika virus infection preferentially counterbalances human peripheral monocyte and/or NK cell activity | |
dc.contributor.author | Lum, F.-M | |
dc.contributor.author | Lee, D | |
dc.contributor.author | Chua, T.-K | |
dc.contributor.author | Tan, J.J | |
dc.contributor.author | Lee, C.Y.P | |
dc.contributor.author | Liu, X | |
dc.contributor.author | Fang, Y | |
dc.contributor.author | Lee, B | |
dc.contributor.author | Yee, W.-X | |
dc.contributor.author | Rickett, N.Y | |
dc.contributor.author | Chia, P.-Y | |
dc.contributor.author | Lim, V | |
dc.contributor.author | Leo, Y.-S | |
dc.contributor.author | Matthews, D.A | |
dc.contributor.author | Hiscox, J.A | |
dc.contributor.author | Nga, L.F.P | |
dc.date.accessioned | 2020-09-14T07:54:56Z | |
dc.date.available | 2020-09-14T07:54:56Z | |
dc.date.issued | 2018 | |
dc.identifier.citation | Lum, F.-M, Lee, D, Chua, T.-K, Tan, J.J, Lee, C.Y.P, Liu, X, Fang, Y, Lee, B, Yee, W.-X, Rickett, N.Y, Chia, P.-Y, Lim, V, Leo, Y.-S, Matthews, D.A, Hiscox, J.A, Nga, L.F.P (2018). Zika virus infection preferentially counterbalances human peripheral monocyte and/or NK cell activity. mSphere 3 (2) : e00120-18. ScholarBank@NUS Repository. https://doi.org/10.1128/mSphereDirect.00120-18 | |
dc.identifier.issn | 2379-5042 | |
dc.identifier.uri | https://scholarbank.nus.edu.sg/handle/10635/176057 | |
dc.description.abstract | Zika virus (ZIKV) has reemerged in the population and caused unprecedented global outbreaks. Here, the transcriptomic consequences of ZIKV infection were studied systematically first in human peripheral blood CD14+ monocytes and monocyte-derived macrophages with high-density RNA sequencing. Analyses of the ZIKV genome revealed that the virus underwent genetic diversification, and differential mRNA abundance was found in host cells during infection. Notably, there was a significant change in the cellular response, with cross talk between monocytes and natural killer (NK) cells as one of the highly identified pathways. Immunophenotyping of peripheral blood from ZIKV-infected patients further confirmed the activation of NK cells during acute infection. ZIKV infection in peripheral blood cells isolated from healthy donors led to the induction of gamma interferon (IFN-γ) and CD107a- two key markers of NK cell function. Depletion of CD14+ monocytes from peripheral blood resulted in a reduction of these markers and reduced priming of NK cells during infection. This was complemented by the immunoproteomic changes observed. Mechanistically, ZIKV infection preferentially counterbalances monocyte and/or NK cell activity, with implications for targeted cytokine immunotherapies. © 2018 Lum et al. | |
dc.source | Unpaywall 20200831 | |
dc.subject | gamma interferon | |
dc.subject | lysosome associated membrane protein 1 | |
dc.subject | transcriptome | |
dc.subject | virus RNA | |
dc.subject | cell culture | |
dc.subject | genetics | |
dc.subject | host pathogen interaction | |
dc.subject | human | |
dc.subject | immunology | |
dc.subject | lymphocyte activation | |
dc.subject | lymphocyte depletion | |
dc.subject | macrophage | |
dc.subject | monocyte | |
dc.subject | natural killer cell | |
dc.subject | proteomics | |
dc.subject | sequence analysis | |
dc.subject | virology | |
dc.subject | virus genome | |
dc.subject | virus replication | |
dc.subject | Zika fever | |
dc.subject | Zika virus | |
dc.subject | Cells, Cultured | |
dc.subject | Genome, Viral | |
dc.subject | Host-Pathogen Interactions | |
dc.subject | Humans | |
dc.subject | Interferon-gamma | |
dc.subject | Killer Cells, Natural | |
dc.subject | Lymphocyte Activation | |
dc.subject | Lymphocyte Depletion | |
dc.subject | Lysosomal-Associated Membrane Protein 1 | |
dc.subject | Macrophages | |
dc.subject | Monocytes | |
dc.subject | Proteomics | |
dc.subject | RNA, Viral | |
dc.subject | Sequence Analysis, RNA | |
dc.subject | Transcriptome | |
dc.subject | Virus Replication | |
dc.subject | Zika Virus | |
dc.subject | Zika Virus Infection | |
dc.type | Article | |
dc.contributor.department | SAW SWEE HOCK SCHOOL OF PUBLIC HEALTH | |
dc.description.doi | 10.1128/mSphereDirect.00120-18 | |
dc.description.sourcetitle | mSphere | |
dc.description.volume | 3 | |
dc.description.issue | 2 | |
dc.description.page | e00120-18 | |
dc.published.state | Published | |
Appears in Collections: | Elements Staff Publications |
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