Please use this identifier to cite or link to this item: https://doi.org/10.1128/mSphereDirect.00120-18
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dc.titleZika virus infection preferentially counterbalances human peripheral monocyte and/or NK cell activity
dc.contributor.authorLum, F.-M
dc.contributor.authorLee, D
dc.contributor.authorChua, T.-K
dc.contributor.authorTan, J.J
dc.contributor.authorLee, C.Y.P
dc.contributor.authorLiu, X
dc.contributor.authorFang, Y
dc.contributor.authorLee, B
dc.contributor.authorYee, W.-X
dc.contributor.authorRickett, N.Y
dc.contributor.authorChia, P.-Y
dc.contributor.authorLim, V
dc.contributor.authorLeo, Y.-S
dc.contributor.authorMatthews, D.A
dc.contributor.authorHiscox, J.A
dc.contributor.authorNga, L.F.P
dc.date.accessioned2020-09-14T07:54:56Z
dc.date.available2020-09-14T07:54:56Z
dc.date.issued2018
dc.identifier.citationLum, F.-M, Lee, D, Chua, T.-K, Tan, J.J, Lee, C.Y.P, Liu, X, Fang, Y, Lee, B, Yee, W.-X, Rickett, N.Y, Chia, P.-Y, Lim, V, Leo, Y.-S, Matthews, D.A, Hiscox, J.A, Nga, L.F.P (2018). Zika virus infection preferentially counterbalances human peripheral monocyte and/or NK cell activity. mSphere 3 (2) : e00120-18. ScholarBank@NUS Repository. https://doi.org/10.1128/mSphereDirect.00120-18
dc.identifier.issn2379-5042
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/176057
dc.description.abstractZika virus (ZIKV) has reemerged in the population and caused unprecedented global outbreaks. Here, the transcriptomic consequences of ZIKV infection were studied systematically first in human peripheral blood CD14+ monocytes and monocyte-derived macrophages with high-density RNA sequencing. Analyses of the ZIKV genome revealed that the virus underwent genetic diversification, and differential mRNA abundance was found in host cells during infection. Notably, there was a significant change in the cellular response, with cross talk between monocytes and natural killer (NK) cells as one of the highly identified pathways. Immunophenotyping of peripheral blood from ZIKV-infected patients further confirmed the activation of NK cells during acute infection. ZIKV infection in peripheral blood cells isolated from healthy donors led to the induction of gamma interferon (IFN-γ) and CD107a- two key markers of NK cell function. Depletion of CD14+ monocytes from peripheral blood resulted in a reduction of these markers and reduced priming of NK cells during infection. This was complemented by the immunoproteomic changes observed. Mechanistically, ZIKV infection preferentially counterbalances monocyte and/or NK cell activity, with implications for targeted cytokine immunotherapies. © 2018 Lum et al.
dc.sourceUnpaywall 20200831
dc.subjectgamma interferon
dc.subjectlysosome associated membrane protein 1
dc.subjecttranscriptome
dc.subjectvirus RNA
dc.subjectcell culture
dc.subjectgenetics
dc.subjecthost pathogen interaction
dc.subjecthuman
dc.subjectimmunology
dc.subjectlymphocyte activation
dc.subjectlymphocyte depletion
dc.subjectmacrophage
dc.subjectmonocyte
dc.subjectnatural killer cell
dc.subjectproteomics
dc.subjectsequence analysis
dc.subjectvirology
dc.subjectvirus genome
dc.subjectvirus replication
dc.subjectZika fever
dc.subjectZika virus
dc.subjectCells, Cultured
dc.subjectGenome, Viral
dc.subjectHost-Pathogen Interactions
dc.subjectHumans
dc.subjectInterferon-gamma
dc.subjectKiller Cells, Natural
dc.subjectLymphocyte Activation
dc.subjectLymphocyte Depletion
dc.subjectLysosomal-Associated Membrane Protein 1
dc.subjectMacrophages
dc.subjectMonocytes
dc.subjectProteomics
dc.subjectRNA, Viral
dc.subjectSequence Analysis, RNA
dc.subjectTranscriptome
dc.subjectVirus Replication
dc.subjectZika Virus
dc.subjectZika Virus Infection
dc.typeArticle
dc.contributor.departmentSAW SWEE HOCK SCHOOL OF PUBLIC HEALTH
dc.description.doi10.1128/mSphereDirect.00120-18
dc.description.sourcetitlemSphere
dc.description.volume3
dc.description.issue2
dc.description.pagee00120-18
dc.published.statePublished
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