Please use this identifier to cite or link to this item: https://doi.org/10.1128/mSphereDirect.00120-18
Title: Zika virus infection preferentially counterbalances human peripheral monocyte and/or NK cell activity
Authors: Lum, F.-M
Lee, D
Chua, T.-K
Tan, J.J
Lee, C.Y.P
Liu, X
Fang, Y
Lee, B
Yee, W.-X
Rickett, N.Y
Chia, P.-Y
Lim, V
Leo, Y.-S 
Matthews, D.A
Hiscox, J.A
Nga, L.F.P
Keywords: gamma interferon
lysosome associated membrane protein 1
transcriptome
virus RNA
cell culture
genetics
host pathogen interaction
human
immunology
lymphocyte activation
lymphocyte depletion
macrophage
monocyte
natural killer cell
proteomics
sequence analysis
virology
virus genome
virus replication
Zika fever
Zika virus
Cells, Cultured
Genome, Viral
Host-Pathogen Interactions
Humans
Interferon-gamma
Killer Cells, Natural
Lymphocyte Activation
Lymphocyte Depletion
Lysosomal-Associated Membrane Protein 1
Macrophages
Monocytes
Proteomics
RNA, Viral
Sequence Analysis, RNA
Transcriptome
Virus Replication
Zika Virus
Zika Virus Infection
Issue Date: 2018
Citation: Lum, F.-M, Lee, D, Chua, T.-K, Tan, J.J, Lee, C.Y.P, Liu, X, Fang, Y, Lee, B, Yee, W.-X, Rickett, N.Y, Chia, P.-Y, Lim, V, Leo, Y.-S, Matthews, D.A, Hiscox, J.A, Nga, L.F.P (2018). Zika virus infection preferentially counterbalances human peripheral monocyte and/or NK cell activity. mSphere 3 (2) : e00120-18. ScholarBank@NUS Repository. https://doi.org/10.1128/mSphereDirect.00120-18
Abstract: Zika virus (ZIKV) has reemerged in the population and caused unprecedented global outbreaks. Here, the transcriptomic consequences of ZIKV infection were studied systematically first in human peripheral blood CD14+ monocytes and monocyte-derived macrophages with high-density RNA sequencing. Analyses of the ZIKV genome revealed that the virus underwent genetic diversification, and differential mRNA abundance was found in host cells during infection. Notably, there was a significant change in the cellular response, with cross talk between monocytes and natural killer (NK) cells as one of the highly identified pathways. Immunophenotyping of peripheral blood from ZIKV-infected patients further confirmed the activation of NK cells during acute infection. ZIKV infection in peripheral blood cells isolated from healthy donors led to the induction of gamma interferon (IFN-γ) and CD107a- two key markers of NK cell function. Depletion of CD14+ monocytes from peripheral blood resulted in a reduction of these markers and reduced priming of NK cells during infection. This was complemented by the immunoproteomic changes observed. Mechanistically, ZIKV infection preferentially counterbalances monocyte and/or NK cell activity, with implications for targeted cytokine immunotherapies. © 2018 Lum et al.
Source Title: mSphere
URI: https://scholarbank.nus.edu.sg/handle/10635/176057
ISSN: 2379-5042
DOI: 10.1128/mSphereDirect.00120-18
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