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https://doi.org/10.1128/mSphereDirect.00120-18
Title: | Zika virus infection preferentially counterbalances human peripheral monocyte and/or NK cell activity | Authors: | Lum, F.-M Lee, D Chua, T.-K Tan, J.J Lee, C.Y.P Liu, X Fang, Y Lee, B Yee, W.-X Rickett, N.Y Chia, P.-Y Lim, V Leo, Y.-S Matthews, D.A Hiscox, J.A Nga, L.F.P |
Keywords: | gamma interferon lysosome associated membrane protein 1 transcriptome virus RNA cell culture genetics host pathogen interaction human immunology lymphocyte activation lymphocyte depletion macrophage monocyte natural killer cell proteomics sequence analysis virology virus genome virus replication Zika fever Zika virus Cells, Cultured Genome, Viral Host-Pathogen Interactions Humans Interferon-gamma Killer Cells, Natural Lymphocyte Activation Lymphocyte Depletion Lysosomal-Associated Membrane Protein 1 Macrophages Monocytes Proteomics RNA, Viral Sequence Analysis, RNA Transcriptome Virus Replication Zika Virus Zika Virus Infection |
Issue Date: | 2018 | Citation: | Lum, F.-M, Lee, D, Chua, T.-K, Tan, J.J, Lee, C.Y.P, Liu, X, Fang, Y, Lee, B, Yee, W.-X, Rickett, N.Y, Chia, P.-Y, Lim, V, Leo, Y.-S, Matthews, D.A, Hiscox, J.A, Nga, L.F.P (2018). Zika virus infection preferentially counterbalances human peripheral monocyte and/or NK cell activity. mSphere 3 (2) : e00120-18. ScholarBank@NUS Repository. https://doi.org/10.1128/mSphereDirect.00120-18 | Abstract: | Zika virus (ZIKV) has reemerged in the population and caused unprecedented global outbreaks. Here, the transcriptomic consequences of ZIKV infection were studied systematically first in human peripheral blood CD14+ monocytes and monocyte-derived macrophages with high-density RNA sequencing. Analyses of the ZIKV genome revealed that the virus underwent genetic diversification, and differential mRNA abundance was found in host cells during infection. Notably, there was a significant change in the cellular response, with cross talk between monocytes and natural killer (NK) cells as one of the highly identified pathways. Immunophenotyping of peripheral blood from ZIKV-infected patients further confirmed the activation of NK cells during acute infection. ZIKV infection in peripheral blood cells isolated from healthy donors led to the induction of gamma interferon (IFN-γ) and CD107a- two key markers of NK cell function. Depletion of CD14+ monocytes from peripheral blood resulted in a reduction of these markers and reduced priming of NK cells during infection. This was complemented by the immunoproteomic changes observed. Mechanistically, ZIKV infection preferentially counterbalances monocyte and/or NK cell activity, with implications for targeted cytokine immunotherapies. © 2018 Lum et al. | Source Title: | mSphere | URI: | https://scholarbank.nus.edu.sg/handle/10635/176057 | ISSN: | 2379-5042 | DOI: | 10.1128/mSphereDirect.00120-18 |
Appears in Collections: | Elements Staff Publications |
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