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Title: Extensively drug-resistant Acinetobacter baumannii in a Thai hospital: A molecular epidemiologic analysis and identification of bactericidal Polymyxin B-based combinations
Authors: Teo, J
Lim, T.-P 
Hsu, L.-Y 
Tan, T.-Y 
Sasikala, S
Hon, P.-Y 
Kwa, A.L 
Apisarnthanarak, A
Keywords: amikacin
piperacillin plus tazobactam
polymyxin B
Acinetobacter baumannii
antibiotic resistance
antibiotic sensitivity
bacterial colonization
bacterial gene
bactericidal activity
blaOXA 23 gene
blaOXA 51 gene
controlled study
in vitro study
infection control
minimum inhibitory concentration
molecular epidemiology
priority journal
Issue Date: 2015
Citation: Teo, J, Lim, T.-P, Hsu, L.-Y, Tan, T.-Y, Sasikala, S, Hon, P.-Y, Kwa, A.L, Apisarnthanarak, A (2015). Extensively drug-resistant Acinetobacter baumannii in a Thai hospital: A molecular epidemiologic analysis and identification of bactericidal Polymyxin B-based combinations. Antimicrobial Resistance and Infection Control 4 (1) : 2. ScholarBank@NUS Repository.
Abstract: Background: Limited knowledge of the local molecular epidemiology and the paucity of new effective antibiotics has resulted in an immense challenge in the control and treatment of extensively drug-resistant (XDR) Acinetobacter baumannii infections in Thailand. Antimicrobial combination regimens may be the only feasible treatment option in such cases. We sought to characterize the local molecular epidemiology and assess the bactericidal activity of various antibiotics individually and in combination against XDR A. baumannii in a Thai hospital. Methods: All XDR A. baumannii isolates from Thammasat University Hospital were collected between October 2010 and May 2011. Susceptibility testing was conducted according to reference broth dilution methods. Pulse-field gel electrophoresis was used to genotype the isolates. Carbapenemase genes were detected using polymerase chain reaction. In vitro testing of clinically-relevant concentrations of imipenem, meropenem, doripenem, rifampicin and tigecycline alone and in combination with polymyxin B was conducted using multiple combination bactericidal testing. Results: Forty-nine polymyxin B-susceptible XDR A. baumannii isolates were identified. bla OXA-23 and bla OXA-51 genes were detected in all isolates. Eight clonally related clusters were identified, resulting in the initiation of several infection control measures. Imipenem, meropenem, doripenem, rifampicin, and tigecycline in combination with PB respectively, exhibited bactericidal killing in 100%, 100%, 98.0%, 100% and 87.8% isolates respectively at 24 hours. Conclusion: Molecular epidemiologic analysis can aid the early detection of infection outbreak within the institution, resulting in the rapid containment of the outbreak. Imipenem/meropenem/rifampicin in combination with polymyxin B demonstrated consistent bactericidal effect against 49 bla OXA-23-harbouring XDR A. baumannii clinical isolates, suggesting a role of combination therapy in the treatment of these infections. © 2015 Teo et al.; licensee BioMed Central.
Source Title: Antimicrobial Resistance and Infection Control
ISSN: 2047-2994
DOI: 10.1186/s13756-015-0043-x
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