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https://doi.org/10.1038/sdata.2015.63
Title: | A resource of ribosomal RNA-depleted RNA-Seq data from different normal adult and fetal human tissues | Authors: | Choy, J.Y.H Boon, P.L.S Bertin, N Fullwood, M.J |
Keywords: | ribosome RNA adult antibody specificity fetus genetics high throughput sequencing human sequence analysis Adult Fetus High-Throughput Nucleotide Sequencing Humans Organ Specificity RNA, Ribosomal Sequence Analysis, RNA |
Issue Date: | 2015 | Citation: | Choy, J.Y.H, Boon, P.L.S, Bertin, N, Fullwood, M.J (2015). A resource of ribosomal RNA-depleted RNA-Seq data from different normal adult and fetal human tissues. Scientific Data 2 : 150063. ScholarBank@NUS Repository. https://doi.org/10.1038/sdata.2015.63 | Abstract: | Gene expression is the most fundamental level at which the genotype leads to the phenotype of the organism. Enabled by ultra-high-throughput next-generation DNA sequencing, RNA-Seq involves shotgun sequencing of fragmented RNA transcripts by next-generation sequencing followed by in silico assembly, and is rapidly becoming the most popular method for gene expression analysis. Poly[A]+ RNA-Seq analyses of normal human adult tissue samples such as Illumina's Human BodyMap 2.0 Project and the RNA-Seq atlas have provided a useful global resource and framework for comparisons with diseased tissues such as cancer. However, these analyses have failed to provide information on poly[A]-RNA, which is abundant in our cells. The most recent advances in RNA-Seq analyses use ribosomal RNA-depletion to provide information on both poly[A]+ and poly[A]-RNA. In this paper, we describe the use of Illumina's HiSeq 2000 to generate high quality rRNA-depleted RNA-Seq datasets from human fetal and adult tissues. The datasets reported here will be useful in understanding the different expression profiles in different tissues. | Source Title: | Scientific Data | URI: | https://scholarbank.nus.edu.sg/handle/10635/175977 | ISSN: | 2052-4463 | DOI: | 10.1038/sdata.2015.63 |
Appears in Collections: | Elements Staff Publications |
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