Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/175919
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dc.titleCHARACTERIZATION OF FERN SPORE ALLERGENS OF SINGAPORE -- WITH SPECIAL REFERENCE TO DICRANOPTERIS SPP
dc.contributor.authorSHANG HUISHEN
dc.date.accessioned2020-09-11T05:19:47Z
dc.date.available2020-09-11T05:19:47Z
dc.date.issued2000
dc.identifier.citationSHANG HUISHEN (2000). CHARACTERIZATION OF FERN SPORE ALLERGENS OF SINGAPORE -- WITH SPECIAL REFERENCE TO DICRANOPTERIS SPP. ScholarBank@NUS Repository.
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/175919
dc.description.abstractSpores from some local ferns were found to contain allergens. Among these, spores from the Dicranopteris linearis and Dicranopteris curranii ferns were significantly more reactive. A 6.5 kDa major allergen from these two fern spores was isolated and identified. Its protein sequence show homology to the 2S seed storage protein. Carbohydrate moiety played an important role in the constitution or its lgE epitopes. Genes encoding this 6.5 kDa allergen from Dicranopteris curranii and Dicranopteris linearis were isolated which contain an open reading frame that defined a 125-residue protein of molecular weight 14611 daltons. The precursor contained a conspicuous signal peptide and sequences of a large and a small subunit. Signal peptide cleavage site and post-translational processing site were confirmed by both nucleic acid and protein sequence analysis as well as enzymatic digestion. The expressed protein could not be recognized by positive sera indicating that the carbohydrate components affected the specific lgE binding to the protein.
dc.sourceCCK BATCHLOAD 20200918
dc.subjectfern
dc.subjectDicranopteris linearis
dc.subjectDicranopteris curranii
dc.subjectallergen
dc.subjectcarbohydrate
dc.subjectseed storage protein
dc.typeThesis
dc.contributor.departmentPAEDIATRICS
dc.contributor.supervisorLEE BEE WAH
dc.description.degreePh.D
dc.description.degreeconferredDOCTOR OF PHILOSOPHY
Appears in Collections:Ph.D Theses (Restricted)

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