Please use this identifier to cite or link to this item: https://doi.org/10.1186/s12936-017-2114-3
Title: Singapore's Anopheles sinensis Form A is susceptible to Plasmodium vivax isolates from the western Thailand-Myanmar border
Authors: Pang, S.-C
Andolina, C
Malleret, B 
Christensen, P.R
Lam-Phua, S.-G
Razak, M.A.B.A
Chong, C.-S
Li, D 
Chu, C.S
Russell, B
Rénia, L 
Ng, L.-C
Nosten, F
Keywords: internal transcribed spacer 2
adult
Anopheles
Anopheles cracens
Anopheles dirus
Anopheles sinensis
Article
clinical article
COI gene
controlled study
female
gene
genetic association
genetic susceptibility
human
informed consent
ITS2 gene
larva
male
Myanmar
nonhuman
oocyst
parasite identification
phylogeny
Plasmodium vivax
Plasmodium vivax malaria
real time polymerase chain reaction
Singapore
sporozoite
Thailand
animal
classification
DNA barcoding
growth, development and aging
mosquito vector
parasitology
physiology
Animals
Anopheles
DNA Barcoding, Taxonomic
Female
Larva
Mosquito Vectors
Myanmar
Plasmodium vivax
Singapore
Thailand
Issue Date: 2017
Citation: Pang, S.-C, Andolina, C, Malleret, B, Christensen, P.R, Lam-Phua, S.-G, Razak, M.A.B.A, Chong, C.-S, Li, D, Chu, C.S, Russell, B, Rénia, L, Ng, L.-C, Nosten, F (2017). Singapore's Anopheles sinensis Form A is susceptible to Plasmodium vivax isolates from the western Thailand-Myanmar border. Malaria Journal 16 (1) : 465. ScholarBank@NUS Repository. https://doi.org/10.1186/s12936-017-2114-3
Abstract: Background: Singapore has been certified malaria-free by the World Health Organization since November 1982. However, sporadic autochthonous malaria outbreaks do occur. In one of the most recent outbreaks of vivax malaria, an entomological investigation identified Anopheles sinensis as the most probable vector. As metaphase karyotype studies divided An. sinensis into two forms, A and B, with different vector competence: the investigation of vector competence of An. sinensis found in Singapore was thus pursued using Plasmodium vivax field isolates from the Thailand-Myanmar border. Methods: Adults and larvae An. sinensis were collected from Singapore from 14 different locations, using various trapping and collection methods between September 2013 and January 2016. Molecular identification of An. sinensis species were conducted by amplifying the ITS2 and CO1 region using PCR. Experimental infections of An. sinensis using blood from seven patients infected with P. vivax from the Thailand-Myanmar border were conducted with Anopheles cracens (An. dirus B) as control. Results: Phylogenetic analysis showed that An. sinensis (F22, F2 and collected from outbreak areas) found in Singapore was entirely Form A, and closely related to An. sinensis Form A from Thailand. Artificial infection of these Singapore strain An. sinensis Form A resulted in the development of oocysts in four experiments, with the number of sporozoites produced by one An. sinensis ranging from 4301 to 14,538. Conclusions: Infection experiments showed that An. sinensis Form A from Singapore was susceptible to Thai-Myanmar P. vivax strain, suggesting a potential role as a malaria vector in Singapore. © 2017 The Author(s).
Source Title: Malaria Journal
URI: https://scholarbank.nus.edu.sg/handle/10635/175418
ISSN: 1475-2875
DOI: 10.1186/s12936-017-2114-3
Appears in Collections:Staff Publications
Elements

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
10_1186_s12936-017-2114-3.pdf2.16 MBAdobe PDF

OPEN

NoneView/Download

SCOPUSTM   
Citations

3
checked on Feb 27, 2021

Page view(s)

28
checked on Mar 5, 2021

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.