Please use this identifier to cite or link to this item: https://doi.org/10.1186/s12879-018-3296-1
Title: Restriction of in vivo infection by antifouling coating on urinary catheter with controllable and sustained silver release: A proof of concept study
Authors: Mandakhalikar, K.D 
Wang, R
Rahmat, J.N 
Chiong, E 
Neoh, K.G 
Tambyah, P.A 
Keywords: silicone
silver nanoparticle
indole derivative
metal nanoparticle
polydopamine
polymer
silver
animal experiment
animal model
antimicrobial activity
Article
bacterial colonization
bacteriuria
bladder
bladder examination
blood analysis
catheter complication
clinical effectiveness
controlled study
drug coating
drug delivery system
encrustation
Escherichia coli
female
in vivo study
minipig
mouse
nonhuman
sustained drug release
urinary tract infection
animal
bacteriuria
biofilm
bladder catheterization
C57BL mouse
catheter infection
chemistry
Escherichia coli infection
human
indwelling catheter
microbiology
pig
proof of concept
urinary catheter
urinary tract infection
Animals
Bacteriuria
Biofilms
Catheter-Related Infections
Catheters, Indwelling
Escherichia coli Infections
Female
Humans
Indoles
Metal Nanoparticles
Mice, Inbred C57BL
Polymers
Proof of Concept Study
Silver
Swine
Swine, Miniature
Urinary Bladder
Urinary Catheterization
Urinary Catheters
Urinary Tract Infections
Issue Date: 2018
Citation: Mandakhalikar, K.D, Wang, R, Rahmat, J.N, Chiong, E, Neoh, K.G, Tambyah, P.A (2018). Restriction of in vivo infection by antifouling coating on urinary catheter with controllable and sustained silver release: A proof of concept study. BMC Infectious Diseases 18 (1) : 370. ScholarBank@NUS Repository. https://doi.org/10.1186/s12879-018-3296-1
Abstract: Background: Catheter Associated Urinary Tract Infections are among the most common urological infections world-wide. Bacterial biofilms and encrustation cause significant complications in patients with urinary catheters. The objective of the study is to demonstrate the efficacy and safety of an anti-microbial and anti-encrustation silver nanoparticle (AgNP) coating on silicone urinary catheter in two different animal models. Methods: Antifouling coating (P3) was prepared with alternate layers of polydopamine and AgNP and an outermost antifouling layer. Sixteen C57BL/6 female mice and two female PWG Micropigs® were used to perform the experiments. In mice, a 5 mm long silicone catheter with or without P3 was transurethrally placed into the urinary bladder. Micropigs were transurethrally implanted - one with P3 silicone catheter and the other with commercially available silver coated silicone catheter. Both models were challenged with E. coli. Bacteriuria was evaluated routinely and upon end of study (2 weeks for mice, 3 weeks for micropigs), blood, catheters and bladders were harvested and analysed for bacterial colonization and encrustation as well as for toxicity. Results: Lower bacterial colonization was seen on P3 catheters as well as in bladders of animals with P3 catheter. Bacteriuria was consistently less in mice with P3 catheter than with uncoated catheters. Encrustation was lower on P3 catheter and in bladder of micropig with P3 catheter. No significant toxicity of P3 was observed in mice or in micropig as compared to controls. The numbers were small in this proof of concept study and technical issues were noted especially with the porcine model. Conclusions: Antifouling P3 coating reduces bacterial colonization on catheter and in animal bladders without causing any considerable toxicity for 2 to 3 weeks. This novel coating could potentially reduce the complications of indwelling urethral catheters. © 2018 The Author(s).
Source Title: BMC Infectious Diseases
URI: https://scholarbank.nus.edu.sg/handle/10635/175374
ISSN: 1471-2334
DOI: 10.1186/s12879-018-3296-1
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