Please use this identifier to cite or link to this item:
https://doi.org/10.1128/IAI.02279-14
DC Field | Value | |
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dc.title | Differential regulation of proinflammatory cytokine expression by mitogen-activated protein kinases in macrophages in response to intestinal parasite infection | |
dc.contributor.author | Lim, M.X | |
dc.contributor.author | Png, C.W | |
dc.contributor.author | Tay, C.Y.B | |
dc.contributor.author | Teo, J.D.W | |
dc.contributor.author | Jiao, H | |
dc.contributor.author | Lehming, N | |
dc.contributor.author | Tan, K.S.W | |
dc.contributor.author | Zhang, Y | |
dc.date.accessioned | 2020-09-09T07:25:27Z | |
dc.date.available | 2020-09-09T07:25:27Z | |
dc.date.issued | 2014 | |
dc.identifier.citation | Lim, M.X, Png, C.W, Tay, C.Y.B, Teo, J.D.W, Jiao, H, Lehming, N, Tan, K.S.W, Zhang, Y (2014). Differential regulation of proinflammatory cytokine expression by mitogen-activated protein kinases in macrophages in response to intestinal parasite infection. Infection and Immunity 82 (11) : 4789-4801. ScholarBank@NUS Repository. https://doi.org/10.1128/IAI.02279-14 | |
dc.identifier.issn | 0019-9567 | |
dc.identifier.uri | https://scholarbank.nus.edu.sg/handle/10635/175313 | |
dc.description.abstract | Blastocystis is a common enteric protistan parasite that can cause acute, as well as chronic, infection and is associated with irritable bowel syndrome (IBS). However, the pathogenic status of Blastocystis infection remains unclear. In this study, we found that Blastocystis antigens induced abundant expression of proinflammatory cytokines, including interleukin 1? (IL-1?), IL-6, and tumor necrosis factor alpha (TNF-?), in mouse intestinal explants, in mouse colitis colon, and in macrophages. Further investigation utilizing RAW264.7 murine macrophages showed that Blastocystis treatment in RAW264.7 macrophages induced the activation of ERK, JNK, and p38, the three major groups of mammalian mitogen-activated protein (MAP) kinases that play essential roles in the expression of proinflammatory cytokines. ERK inhibition in macrophages significantly suppressed both mRNA and protein expression of IL-6 and TNF-? and mRNA expression of IL-1?. On the other hand, JNK inhibition resulted in reductions in both c-Jun and ERK activation and significant suppression of all three proinflammatory cytokines at both the mRNA and protein levels. Inhibition of p38 suppressed only IL-6 protein expression with no effect on the expression of IL-1? and TNF-?. Furthermore, we found that serine proteases produced by Blastocystis play an important role in the induction of ERK activation and proinflammatory cytokine expression by macrophages. Our study thus demonstrated for the first time that Blastocystis could induce the expression of various proinflammatory cytokines via the activation of MAP kinases and that infection with Blastocystis may contribute to the pathogenesis of inflammatory intestinal diseases through the activation of inflammatory pathways in host immune cells, such as macrophages. © 2014, American Society for Microbiology. | |
dc.publisher | American Society for Microbiology | |
dc.source | Unpaywall 20200831 | |
dc.subject | interleukin 1beta | |
dc.subject | interleukin 6 | |
dc.subject | Janus kinase | |
dc.subject | mitogen activated protein kinase | |
dc.subject | mitogen activated protein kinase p38 | |
dc.subject | tumor necrosis factor alpha | |
dc.subject | cytokine | |
dc.subject | mitogen activated protein kinase kinase | |
dc.subject | animal cell | |
dc.subject | animal experiment | |
dc.subject | animal model | |
dc.subject | Article | |
dc.subject | blastocystosis | |
dc.subject | cell viability | |
dc.subject | controlled study | |
dc.subject | cytokine response | |
dc.subject | enzyme activation | |
dc.subject | enzyme inhibition | |
dc.subject | enzyme phosphorylation | |
dc.subject | host pathogen interaction | |
dc.subject | intestine cell | |
dc.subject | intestine epithelium | |
dc.subject | macrophage | |
dc.subject | male | |
dc.subject | mouse | |
dc.subject | nonhuman | |
dc.subject | pathogenesis | |
dc.subject | protein expression | |
dc.subject | protein function | |
dc.subject | regulatory mechanism | |
dc.subject | signal transduction | |
dc.subject | upregulation | |
dc.subject | animal | |
dc.subject | antagonists and inhibitors | |
dc.subject | Blastocystis | |
dc.subject | blastocystosis | |
dc.subject | cell line | |
dc.subject | gene expression regulation | |
dc.subject | genetics | |
dc.subject | immunology | |
dc.subject | macrophage | |
dc.subject | metabolism | |
dc.subject | tissue culture technique | |
dc.subject | Animals | |
dc.subject | Blastocystis | |
dc.subject | Blastocystis Infections | |
dc.subject | Cell Line | |
dc.subject | Cytokines | |
dc.subject | Gene Expression Regulation | |
dc.subject | Macrophages | |
dc.subject | Mice | |
dc.subject | Mitogen-Activated Protein Kinase Kinases | |
dc.subject | Tissue Culture Techniques | |
dc.type | Article | |
dc.contributor.department | MICROBIOLOGY AND IMMUNOLOGY | |
dc.description.doi | 10.1128/IAI.02279-14 | |
dc.description.sourcetitle | Infection and Immunity | |
dc.description.volume | 82 | |
dc.description.issue | 11 | |
dc.description.page | 4789-4801 | |
dc.published.state | Published | |
Appears in Collections: | Staff Publications Elements |
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