Please use this identifier to cite or link to this item: https://doi.org/10.1128/IAI.02279-14
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dc.titleDifferential regulation of proinflammatory cytokine expression by mitogen-activated protein kinases in macrophages in response to intestinal parasite infection
dc.contributor.authorLim, M.X
dc.contributor.authorPng, C.W
dc.contributor.authorTay, C.Y.B
dc.contributor.authorTeo, J.D.W
dc.contributor.authorJiao, H
dc.contributor.authorLehming, N
dc.contributor.authorTan, K.S.W
dc.contributor.authorZhang, Y
dc.date.accessioned2020-09-09T07:25:27Z
dc.date.available2020-09-09T07:25:27Z
dc.date.issued2014
dc.identifier.citationLim, M.X, Png, C.W, Tay, C.Y.B, Teo, J.D.W, Jiao, H, Lehming, N, Tan, K.S.W, Zhang, Y (2014). Differential regulation of proinflammatory cytokine expression by mitogen-activated protein kinases in macrophages in response to intestinal parasite infection. Infection and Immunity 82 (11) : 4789-4801. ScholarBank@NUS Repository. https://doi.org/10.1128/IAI.02279-14
dc.identifier.issn0019-9567
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/175313
dc.description.abstractBlastocystis is a common enteric protistan parasite that can cause acute, as well as chronic, infection and is associated with irritable bowel syndrome (IBS). However, the pathogenic status of Blastocystis infection remains unclear. In this study, we found that Blastocystis antigens induced abundant expression of proinflammatory cytokines, including interleukin 1? (IL-1?), IL-6, and tumor necrosis factor alpha (TNF-?), in mouse intestinal explants, in mouse colitis colon, and in macrophages. Further investigation utilizing RAW264.7 murine macrophages showed that Blastocystis treatment in RAW264.7 macrophages induced the activation of ERK, JNK, and p38, the three major groups of mammalian mitogen-activated protein (MAP) kinases that play essential roles in the expression of proinflammatory cytokines. ERK inhibition in macrophages significantly suppressed both mRNA and protein expression of IL-6 and TNF-? and mRNA expression of IL-1?. On the other hand, JNK inhibition resulted in reductions in both c-Jun and ERK activation and significant suppression of all three proinflammatory cytokines at both the mRNA and protein levels. Inhibition of p38 suppressed only IL-6 protein expression with no effect on the expression of IL-1? and TNF-?. Furthermore, we found that serine proteases produced by Blastocystis play an important role in the induction of ERK activation and proinflammatory cytokine expression by macrophages. Our study thus demonstrated for the first time that Blastocystis could induce the expression of various proinflammatory cytokines via the activation of MAP kinases and that infection with Blastocystis may contribute to the pathogenesis of inflammatory intestinal diseases through the activation of inflammatory pathways in host immune cells, such as macrophages. © 2014, American Society for Microbiology.
dc.publisherAmerican Society for Microbiology
dc.sourceUnpaywall 20200831
dc.subjectinterleukin 1beta
dc.subjectinterleukin 6
dc.subjectJanus kinase
dc.subjectmitogen activated protein kinase
dc.subjectmitogen activated protein kinase p38
dc.subjecttumor necrosis factor alpha
dc.subjectcytokine
dc.subjectmitogen activated protein kinase kinase
dc.subjectanimal cell
dc.subjectanimal experiment
dc.subjectanimal model
dc.subjectArticle
dc.subjectblastocystosis
dc.subjectcell viability
dc.subjectcontrolled study
dc.subjectcytokine response
dc.subjectenzyme activation
dc.subjectenzyme inhibition
dc.subjectenzyme phosphorylation
dc.subjecthost pathogen interaction
dc.subjectintestine cell
dc.subjectintestine epithelium
dc.subjectmacrophage
dc.subjectmale
dc.subjectmouse
dc.subjectnonhuman
dc.subjectpathogenesis
dc.subjectprotein expression
dc.subjectprotein function
dc.subjectregulatory mechanism
dc.subjectsignal transduction
dc.subjectupregulation
dc.subjectanimal
dc.subjectantagonists and inhibitors
dc.subjectBlastocystis
dc.subjectblastocystosis
dc.subjectcell line
dc.subjectgene expression regulation
dc.subjectgenetics
dc.subjectimmunology
dc.subjectmacrophage
dc.subjectmetabolism
dc.subjecttissue culture technique
dc.subjectAnimals
dc.subjectBlastocystis
dc.subjectBlastocystis Infections
dc.subjectCell Line
dc.subjectCytokines
dc.subjectGene Expression Regulation
dc.subjectMacrophages
dc.subjectMice
dc.subjectMitogen-Activated Protein Kinase Kinases
dc.subjectTissue Culture Techniques
dc.typeArticle
dc.contributor.departmentMICROBIOLOGY AND IMMUNOLOGY
dc.description.doi10.1128/IAI.02279-14
dc.description.sourcetitleInfection and Immunity
dc.description.volume82
dc.description.issue11
dc.description.page4789-4801
dc.published.statePublished
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