Please use this identifier to cite or link to this item: https://doi.org/10.1373/clinchem.2015.252932
Title: High-sensitivity sandwich ELISA for plasma NT-proUcn2: Plasma concentrations and relationship to mortality in heart failure
Authors: Liew, O.W 
Yandle, T.G
Chong, J.P.C 
Ng, Y.X 
Frampton, C.M
Ng, T.P 
Lam, C.S.P 
Richards, A.M 
Keywords: amino terminal pro brain natriuretic peptide
troponin T
urocortin II
biological marker
corticotropin releasing factor
UCN2 protein, human
urocortin
adult
Article
blood level
body mass
cardiovascular mortality
chemoluminescence
comparative study
controlled study
Doppler echocardiography
enzyme linked immunosorbent assay
female
glomerulus filtration rate
heart failure
heart left ventricle ejection fraction
human
limit of detection
major clinical study
male
surface plasmon resonance
aged
blood
heart failure
luminescence
middle aged
mortality
very elderly
Adult
Aged
Aged, 80 and over
Biomarkers
Corticotropin-Releasing Hormone
Enzyme-Linked Immunosorbent Assay
Female
Heart Failure
Humans
Luminescence
Male
Middle Aged
Urocortins
Issue Date: 2016
Publisher: American Association for Clinical Chemistry Inc.
Citation: Liew, O.W, Yandle, T.G, Chong, J.P.C, Ng, Y.X, Frampton, C.M, Ng, T.P, Lam, C.S.P, Richards, A.M (2016). High-sensitivity sandwich ELISA for plasma NT-proUcn2: Plasma concentrations and relationship to mortality in heart failure. Clinical Chemistry 62 (6) : 856-865. ScholarBank@NUS Repository. https://doi.org/10.1373/clinchem.2015.252932
Abstract: BACKGROUND: Urocortin 2 (Ucn2) has powerful hemodynamic, renal, and neurohormonal actions and likely participates in normal circulatory homeostasis and the compensatory response to heart failure (HF). A validated assay for endogenous circulating Ucn2 would facilitate investigations into Ucn2 physiology and elucidate its derangement and potential as a biomarker in heart disease. METHOD: We developed a chemiluminescence-based sandwich ELISA to measure plasma N-terminal (NT)-proUcn2 in non-HF patients (control; n = 160) and HF patients with reduced (HFREF; n = 134) and preserved (HFPEF; n = 121) left ventricular ejection fraction (LVEF). RESULTS: The ELISA had a limit of detection of 8.47 ng/L (1.52 pmol/L) and working range of 23.8-572 ng/L. Intra- and interassay CV and total error were 4.8, 16.2, and 17.7%, respectively. The median (interquartile range) plasma NT-proUcn2 concentration in controls was 112 (86-132) ng/L. HFREF, HFPEF, and all HF plasma concentrations were significantly increased [117 (98-141) ng/L, P = 0.0007; 119 (93-136) ng/L, P = 0.0376, and 119 (97-140) ng/L, P = 0.001] compared with controls but did not differ significantly between HFREF and HFPEF. NT-proUcn2 was modestly related to age (r = 0.264, P = 0.001) and cardiac troponin T (r = 0.258, P = 0.001) but not N-terminal pro-B-type natriuretic peptide, body mass index, LVEF, or estimated glomerular filtration rate. On multivariate analysis, plasma NT-proUcn2 was independently and inversely related to 2-year mortality in HF. CONCLUSIONS: The validated ELISA measured human NT-proUcn2 in plasma and showed modest but significant increases in HF patients compared with controls. In HF, the unusual inverse relationship between plasma NT-proUcn2 and 2-year mortality portends potential prognostic value but requires further corroboration. © 2016 American Association for Clinical Chemistry.
Source Title: Clinical Chemistry
URI: https://scholarbank.nus.edu.sg/handle/10635/175254
ISSN: 0009-9147
DOI: 10.1373/clinchem.2015.252932
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