Please use this identifier to cite or link to this item: https://doi.org/10.1007/s12035-017-0784-z
DC FieldValue
dc.titleEnriched Expression of Neutral Sphingomyelinase 2 in the Striatum is Essential for Regulation of Lipid Raft Content and Motor Coordination
dc.contributor.authorTan, L.H.-R
dc.contributor.authorTan, A.J.-R
dc.contributor.authorNg, Y.-Y
dc.contributor.authorChua, J.J.-E
dc.contributor.authorChew, W.-S
dc.contributor.authorMuralidharan, S
dc.contributor.authorTorta, F
dc.contributor.authorDutta, B
dc.contributor.authorSze, S.K
dc.contributor.authorHerr, D.R
dc.contributor.authorOng, W.-Y
dc.date.accessioned2020-09-09T03:46:35Z
dc.date.available2020-09-09T03:46:35Z
dc.date.issued2018
dc.identifier.citationTan, L.H.-R, Tan, A.J.-R, Ng, Y.-Y, Chua, J.J.-E, Chew, W.-S, Muralidharan, S, Torta, F, Dutta, B, Sze, S.K, Herr, D.R, Ong, W.-Y (2018). Enriched Expression of Neutral Sphingomyelinase 2 in the Striatum is Essential for Regulation of Lipid Raft Content and Motor Coordination. Molecular Neurobiology 55 (7) : 5741-5756. ScholarBank@NUS Repository. https://doi.org/10.1007/s12035-017-0784-z
dc.identifier.issn0893-7648
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/175111
dc.description.abstractSphingomyelinases are a family of enzymes that hydrolyze sphingomyelin to generate phosphocholine and ceramide. The brain distribution and function of neutral sphingomyelinase 2 (nSMase2) were elucidated in this study. nSMase2 mRNA expression was greatest in the striatum, followed by the prefrontal cortex, hippocampus, cerebellum, thalamus, brainstem, and olfactory bulb. The striatum had the highest level of nSMase2 protein expression, followed by the prefrontal cortex, thalamus, hippocampus, brainstem, and cerebellum. Dense immunolabeling was observed in the striatum, including the caudate-putamen, while moderately dense staining was found in the olfactory bulb and cerebral neocortex. Electron microscopy of the caudate-putamen showed nSMase2 immunoreaction product was present in small diameter dendrites or dendritic spines, that formed asymmetrical synapses with unlabeled axon terminals containing small round vesicles; and characteristics of glutamatergic axons. Lipidomic analysis of the striatum showed increase in long chain sphingomyelins, SM36:1 and SM38:1 after inhibition of nSMase activity. Quantitative proteomic analysis of striatal lipid raft fraction showed many proteins were downregulated by more than 2-fold after inhibition or antisense knockdown of nSMase; consistent with the notion that nSMase2 activity is important for aggregation or clustering of proteins in lipid rafts. Inhibition or antisense knockdown of nSMase2 in the caudate-putamen resulted in motor deficits in the rotarod and narrow beam tests; as well as decreased acoustic startle and improved prepulse inhibition of the startle reflex. Together, results indicate an important function of nSMase2 in the striatum. © 2017, The Author(s).
dc.publisherHumana Press Inc.
dc.sourceUnpaywall 20200831
dc.subjectmessenger RNA
dc.subjectneutral sphingomyelinase 2
dc.subjectsphingomyelin phosphodiesterase
dc.subjectunclassified drug
dc.subjectisoenzyme
dc.subjectmessenger RNA
dc.subjectproteome
dc.subjectsphingolipid
dc.subjectsphingomyelin phosphodiesterase
dc.subjectadult
dc.subjectanimal cell
dc.subjectanimal experiment
dc.subjectanimal tissue
dc.subjectantibody labeling
dc.subjectArticle
dc.subjectbrain cortex
dc.subjectbrain stem
dc.subjectcerebellum
dc.subjectcontrolled study
dc.subjectcorpus striatum
dc.subjectdendritic spine
dc.subjectdorsal striatum
dc.subjectdown regulation
dc.subjectelectron microscopy
dc.subjectenzyme activity
dc.subjectenzyme inhibition
dc.subjectgene expression
dc.subjectglutamatergic synapse
dc.subjecthippocampus
dc.subjectlipid analysis
dc.subjectlipid metabolism
dc.subjectlipid raft
dc.subjectlipidomics
dc.subjectmale
dc.subjectmotor coordination
dc.subjectnerve ending
dc.subjectnonhuman
dc.subjectolfactory bulb
dc.subjectprefrontal cortex
dc.subjectprotein expression
dc.subjectproteomics
dc.subjectquantitative analysis
dc.subjectrat
dc.subjectrotarod test
dc.subjectthalamus
dc.subjectacoustic reflex
dc.subjectanimal
dc.subjectcorpus striatum
dc.subjectcytology
dc.subjectenzymology
dc.subjectgene expression regulation
dc.subjectgene knockdown
dc.subjectgenetics
dc.subjectmembrane microdomain
dc.subjectmetabolism
dc.subjectmotor activity
dc.subjectprepulse inhibition
dc.subjectstartle reflex
dc.subjectultrastructure
dc.subjectWistar rat
dc.subjectAnimals
dc.subjectCorpus Striatum
dc.subjectGene Expression Regulation, Enzymologic
dc.subjectGene Knockdown Techniques
dc.subjectIsoenzymes
dc.subjectMale
dc.subjectMembrane Microdomains
dc.subjectMotor Activity
dc.subjectPrepulse Inhibition
dc.subjectProteome
dc.subjectRats, Wistar
dc.subjectReflex, Acoustic
dc.subjectReflex, Startle
dc.subjectRNA, Messenger
dc.subjectRotarod Performance Test
dc.subjectSphingolipids
dc.subjectSphingomyelin Phosphodiesterase
dc.typeArticle
dc.contributor.departmentDEPT OF PHYSIOLOGY
dc.contributor.departmentDEPT OF PHARMACOLOGY
dc.contributor.departmentDEPT OF MEDICINE
dc.contributor.departmentDEPT OF BIOCHEMISTRY
dc.contributor.departmentDEPT OF ANATOMY
dc.description.doi10.1007/s12035-017-0784-z
dc.description.sourcetitleMolecular Neurobiology
dc.description.volume55
dc.description.issue7
dc.description.page5741-5756
dc.published.statePublished
Appears in Collections:Staff Publications
Elements

Show simple item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
10_1007_s12035-017-0784-z.pdf2.28 MBAdobe PDF

OPEN

NoneView/Download

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.