Please use this identifier to cite or link to this item: https://doi.org/10.1242/bio.033753
Title: Cell-autonomous role of GFR?1 in the development of olfactory bulb GABAergic interneurons
Authors: Zechel, S
Fernandez-Suarez, D
Ibáñez, C.F 
Keywords: cre recombinase
glial cell line derived neurotrophic factor
glutamate decarboxylase 67
green fluorescent protein
growth factor receptor alpha 1
tamoxifen
unclassified drug
adult
animal experiment
Article
cell differentiation
cell migration
controlled study
embryo development
female
GABAergic system
immunohistochemistry
interneuron
male
mammal
mouse
neuroblast
newborn
nonhuman
olfactory bulb
olfactory receptor neuron
primordium
protein expression
Issue Date: 2018
Citation: Zechel, S, Fernandez-Suarez, D, Ibáñez, C.F (2018). Cell-autonomous role of GFR?1 in the development of olfactory bulb GABAergic interneurons. Biology Open 7 (5) : bio033753.. ScholarBank@NUS Repository. https://doi.org/10.1242/bio.033753
Abstract: GFR?1, a receptor for glial cell line-derived neurotrophic factor (GDNF), is critical for the development of the main olfactory system. The olfactory bulb (OB) of Gfra1 knockout mice shows significant reductions in the number of olfactory sensory neurons, mitral and tufted cells, as well as all major classes of OB GABAergic interneurons. However, the latter do not express significant levels of GFR?1, leaving the mechanism of action of GFR?1 in OB interneuron development unexplained. Here we report that GFR?1 is highly expressed in the precursor cells that give rise to all major classes of OB interneurons, but is downregulated as these neurons mature. Conditional ablation of GFR?1 in embryonic GABAergic cells recapitulated the cell losses observed in global Gfra1 knockouts at birth. GFR?1 was also required for the sustained generation and allocation of OB interneurons in adulthood. Conditional loss of GFR?1 altered the migratory behaviour of neuroblasts along the rostral migratory stream (RMS) as well as RMS glial tunnel formation. Together, these data indicate that GFR?1 functions cell-autonomously in subpopulations of OB interneuron precursors to regulate their generation and allocation in the mammalian OB. © 2018. Published by The Company of Biologists Ltd.
Source Title: Biology Open
URI: https://scholarbank.nus.edu.sg/handle/10635/175076
ISSN: 20466390
DOI: 10.1242/bio.033753
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